468 



APPENDIX. 



Strength. 



85 

 per cent. 



70 

 per cent. 



Made approximately as under. 



Made by taking 100 vols. 

 90 per cent, alcohol, 

 and 6'& vols. water. 



May be made of 100 vols. 

 90 per cent, alcohol 

 or methylated spirit, 

 and 31 vols. water ; or 

 100 vols. 85 per cent, 

 alcohol, and 23 vols. 

 water. 



50 

 per cent. 



" One- 

 third 

 alcohol" 



Made by taking 100 vols. 



90 per cent, alcohol, 



and 84' 7 vols. water; 



or 100 vols. 70 per cent. 



alcohol, and 41'7 vols. 



water. 

 Made by taking 1 vol. of 



90 per cent, alcohol, 



and 2 vols. water. 



Observations. 



Eectified Spirit B.P. is a 

 little weaker than this, 

 viz. 84*5 per cent. 



Only exceptionally power- 

 ful clearers, such as 

 anilin oil, will clear 

 from this grade ; see 

 353. This is the proper 

 grade in general for 

 preserving organisms 

 and tissues in (but see 

 p. 3 sub. fin.) ; higher 

 gradesshould notgene- 

 rallyjbe used unless it 

 is desired to harden. 

 This is the proper 

 grade for washing out 

 borax-carmine stains, 

 corrosive sublimate 

 after fixing, &c. 



This is the strength of 

 Proof Spirit. 



See G^. 



863, Histological Reagents and Apparatus. The great 

 importance of working with trustworthy chemicals has been 

 insisted on in 94, where also the addresses of some chemists 

 who make a speciality of histological reagents have been 

 given. As regards Dr. GRUBLEE'S products, so often quoted 

 in the foregoing pages, I would add that they should either 

 be ordered from him direct (address, Herrn Dr. G. Grubler, 



