APPENDIX. 475 



heated on the slide, with the section in it, until it imme- 

 diately sets hard on cooling. 



878. Methylen Blue for Impregnation Purposes. APATHY has a 

 note in Zeit. f. wiss. Mik., ix, 4, 1893, p. 466, to the effect that the best 

 methylen blue for impregnation in fact, the only one that will give exactly 

 the results described by him (see 118) is that obtained from E. MERCK, 

 of Darmstadt, and quoted in his price list as " medicinisches Methylenblau," 

 and described on the label as " Anilin-blau, Methylen, chemisch rein und 

 chlorzinkfrei." 



879. Mounting Methyl Green Stains. Attention was called 

 in 109 to the difficulty of mounting methyl green stains in 

 balsam on account of the imperfect resistance of the colour 

 to alcohol. SQUIRE says, in his Methods and Formulse, p. 36, 

 that a thorough washing with water previous to the treat- 

 ment with alcohol has the effect of fixing the colour in the 

 nuclei, so that it is less easily removed by the spirit. 



880. GOODALL'S Hapid Method for Preparing Spinal Cord (Brit. 

 Med. Journ., May, 1893, p. 947 ; Journ. Boy. Mic. Soc., 1893, p. 405). 

 Cut sections of fresh tissue with a freezing microtome ; float them on to 

 water, and as soon as possible drain and float them on to pyridin. After a 

 quarter of an hour wash in water ; stain with 0'25 per cent, aqueous solu- 

 tion of anilin blue-black, followed by picro-carmine ; dehydrate and clear 

 in pyridin ; mount in balsam thinned with pyridin. See also ante, 90. 



881. Demonstration of Living Trichinae (BABNES, Amer. Mon. Mic. 

 Journ., xiv, 1893, p. 104 ; Journ. Roy. Mic. Soc., 1893, p. 406). A piece 

 of trichinised muscle of the size of a pea should be placed in a bottle in a 

 mixture of 3 gr. of pepsin, 2 dr. of water, and 2 minims of hydrochloric 

 acid. The whole should be kept at body temperature for about three hours 

 with occasional shaking. The flesh and cysts being dissolved, the fluid is 

 poured into a conical giass, and allowed to settle ; the trichinae are drawn 

 off from the bottom with a pipette, got on to a slide with water, and 

 examined on a hot stage. 



