AGARIC US. 101 



they are more closely packed towards the periphery 

 than near the centre of the section. 



Examining the sections under a high power it will 

 further be observed 



1. That the hyphae are branched, while occasionally 

 their endings are to be seen. 



2. That they are thin-walled, the transverse cr oblique 

 septa being so disposed that the cells are not much 

 longer than broad. 



3. Here and there are to be found hyphse with fewer 

 septa, and highly refractive contents. 



4. The protoplasmic contents of the hyphae which 

 make up the bulk of the tissue are far from being 

 copious, while no single, well-marked nucleus is to be 

 found in the individual cells. 



By careful staining it is possible to demonstrate the presence of 

 numerous very small nuclei in the protoplasm of the hyphse. 

 Strasburger recommends A. pratensis as a more suitable object for 

 this demonstration than A. campestris. If fresh material be used, 

 the sections may be stained with methyl-green in 1 per cent, 

 acetic acid, washed with 1 per cent, acetic acid, and mounted in 

 weak glycerine and acetic acid ; if chromic acid material be used, 

 it should be carefully washed from the acid, and be stained with 

 hsematoxylin. 



VI. Cut transverse sections of the stipe, and, treating 

 as before, observe that the hyphse appear circular in 

 section, that they are more loosely packed towards the 

 centre than at the periphery, and that throughout inter- 

 cellular spaces are to be recognised. 



Observe under a high power that where the section 

 includes one of the septa (which will thus be seen in 

 surface view) a central highly refractive spot is to be 



