6 CONTENTS 



PAGE 



CHAPTER XIX. BACTERIOLYSINS 358 



Historic 358 Definition, 359 Origin of bacteriolysins, 360 Leukins and 

 leukocytic extracts, 360 Method of preparing leukocytic extracts 361 Mecn- 

 anism of bacteriolysis, 362 General properties of bacteriolysms 363 Normal 

 bacteriolysins, 363 Specificity of bacteriolysms, 363. Practical Applications, 

 36 3_Technic of the Pfeiffer test, 364 Bacteriolytic test in vivo for the identi- 

 fication of bacteria, 365 Bacteriolytic test in vivo in the diagnosis of disease, 

 370_Bacteriolytic test in vitro (method of Stem and Korte), 371 Bacterio- 

 lytic test in vitro (method of Wright), 377. 



CHAPTER XX. HEMOLYSINS 383 



Historic, 383 Definition, 384 Nomenclature, 385 Nature of hemolysins, 

 385_Analogy between bacteriolysis and hemolysis, 389 Specificity of hemoly- 

 sins, 390 Normal hemolysins, 390 Production of immune hemolysins, 393 

 General properties of hemolysins, 393 Source of hemolysins, 394. Practical 

 Applications, 395 Quantitative reactions between hemolytic amboceptor and 

 complement, 396 Method of titration of hemolysin, 397 Method for removing 

 hemolysin from a serum, 400 Method of determining natural hemolysins in 

 serum, 400 The serum diagnosis of paroxysmal hemoglobinuria, 401 Method 

 of determining the resistance of red blood-corpuscles, 402. 



CHAPTER XXI. VENOM HEMOLYSIS. 405 



Historic nature of venom hemolysis, 405^ Venom hemolysis in syphilis, 

 407 Practical value of the venom test in syphilis, 410 The psycho-reaction of 

 Much in syphilis, 410 Venom hemolysis in tuberculosis, 412 Venom hemolysis 

 in cancer, 412. 



CHAPTER XXII. PRINCIPLES OF THE PHENOMENON OF COMPLEMENT FIXATION 413 



Historic, 413 The original complement-fixation method of Bordet, 416 

 Mechanism of complement fixation, 417 Non-specific complement fixation, 

 418 Quantitative factors in complement-fixation tests, 421 Practical applica- 

 tions, 423. 



CHAPTER XXIII. THE TECHNIC OF COMPLEMENT-FIXATION REACTIONS 425 



The Wassermann Syphilis Reaction, 425 Historic, 425 Principles and 

 theories of the syphilis reaction, 428 General technic, 431 Preparation of the 

 fluid to be tested, 432 Preparation and titration of complement, 435 Preserva- 

 tion of complement, 437 Preparation and titration of hemolytic amboceptor, 

 439^ Preparation of blood-corpuscles, 440 Preparation and standardization of 

 antigens, 441 Technic of the First Method, 459 Technic of the Second Method, 

 465- r Technic of the Third Method, 467 Technic of the Fourth Method, 470. 

 Modifications of the Wassermann Reaction, 475 Technic of the Noguchi modi- 

 fication, 476 Technic of the Hecht-Gradwohl modification, 482 Other modi- 

 fications, 484. The Wassermann Reactions in the Various Stages of Syphilis, 

 485 The specificity of the Wassermann reaction^ 490 The effect of treatment 

 upon the Wassermann reaction, 492 The Practical Value of the Wassermann 

 reaction, 495. 



CHAPTER XXIV. COMPLEMENT-FIXATION REACTIONS (continued) 498 



Specific complement fixation in bacterial diseases, 498 Preparation of 

 bacterial antigens, 498 Standardizing bacterial antigens, 500 Principles of 

 complement fixation with bacterial antigens, 501. Complement Fixation in the 

 Differentiation of Microparasites, 502. Complement Fixation in Gonococcus 

 Infections, 503. Complement Fixation in Glanders, 511. Complement Fixa- 

 tion in Contagious Abortion, 513. Complement Fixation in Dourine, 515. 

 Complement Fixation in Typhoid Fever, 516. Complement Fixation in Tuber- 

 culosis, 517. Complement Fixation in Pertussis, 521. Complement Fixation 

 in the Standardization of Immune Serums, 524. Complement Fixation in 

 Echinococcus Disease, 524. Complement Fixation in the Differentiation of 

 Proteins (blood-stains, meats, bacteria), 527. Complement Fixation in Cancer, 



