MODIFICATIONS OF THE WASSERMANN REACTION 



481 



place five drops (0.1 c.c.) of antigen emulsion (alcoholic solution, 1 part, 

 with saline solution, 9 parts); then add five drops (0.1 c.c.) of com- 

 plement (40 per cent.) to all the tubes. Into each of the first pair of 

 tubes place one drop (0.02 c.c.) of active or four drops (0.08 c.c.) of 

 inactivated patient's serum, and mark the front tube with the patient's 

 name. To each of the second pair of tubes add an equal amount of 

 syphilitic serum known to give a positive reaction (positive control), and 

 to each of the third pair add normal serum known to give a negative re- 

 action (negative control) . Mark the tubes in the front row of each pair 

 respectively. The front tube of the fourth pair is the antigen control, 

 and the rear tube the hemolytic control, and each should be so labeled. 

 Into each tube place 1 c.c. of the 1 per cent, corpuscle suspension and 1 

 c.c. of saline solution, making the total volume in each tube about 2 c.c. 

 Shake each tube and incubate at 37 C. for one hour (half an hour in the 

 water-bath). At the end of this time add two units of amboceptor to 

 each tube, shake gently, and reincubate for two hours (one hour in the 

 water-bath). During this time the tubes should be shaken gently 

 once or twice to break up any masses of agglutinated corpuscles. 



The following chart, after Noguchi, illustrates the various steps to 

 be taken in making the test with one patient's serum. Of course, any 

 number of serums may be examined with the same controls (Fig. 119). 



TABLE 16. NOGUCHI MODIFICATION OF THE WASSERMANN 



REACTION 



31 



Four drops if serum is inactivated. 



