ANAPHYLAXIS 941 



(b) Give three young guinea-pigs an intraperitoneal injection of 0.01- 

 c.c. of human serum. 



(c) Give two young guinea-pigs an intraperitoneal injection of 0.1 

 c.c. egg-albumen (1 c.c. of 1 : 10 dilution). 



(d) Give two rabbits 1 c.c. of horse serum intravenously every three 

 days for three doses. 



(e) Give a dog 10 c.c. of horse serum subcutaneously. 



EXPERIMENT 107. ANAPHYLAXIS IN THE GUINEA-PIG. SPECIFICITY 

 OF ANAPHYLAXIS 



1. Two weeks after the sensitizing injections proceed as follows: 



(a) Inject a guinea-pig sensitized to horse serum with 1 c.c. horse serum intra- 

 peritoneally. 



(b) Inject a guinea-pig sensitized to human serum with 0.1 c.c. of human serum 

 intravenously. 



(c) Inject a guinea-pig sensitized to egg-albumen with 1 c.c. of diluted (1 : 4) 

 albumin intraperitoneally. 



(d) Inject a guinea-pig sensitized to horse serum with 1 c.c. of diluted (1 : 4) egg- 

 albumen. 



(e) Inject a guinea-pig sensitized to human serum with 0.1 c.c. horse serum 

 intravenously . 



(f) Inject a guinea-pig sensitized to horse serum with 1 c.c. human serum intra- 

 peritoneally. 



2. Carefully study the symptoms of anaphylaxis. After death autopsy the 

 animals. 



(a) Describe acute anaphylactic shock in the guinea-pig. Does 

 death always occur? 



(b) How soon after the intraperitoneal injection of the intoxicating 

 dose do symptoms develop? After the intravenous? Why the differ- 

 ence? 



(c) Is anaphylaxis a specific reaction? 



(d) What are anaphylactogens? 



(e) Discuss the prevailing views regarding the mechanism of the 

 anaphylactic reaction. 



(f) Describe the anatomic changes occurring in acute and fatal 

 anaphylaxis in the guinea-pig. Discuss the cause and nature of these 

 changes. 



EXERCISE 55. ANAPHYLAXIS (Continued) 

 EXPERIMENT 108. NATURE OF THE ANAPHYLATOXIN 



1. Place 15 grams of coli bacterial substance prepared as described on page 128 

 hi a distilling flask and add 250 c.c. of a 2 per cent, caustic soda in absolute ethyl 

 alcohol. Place on a water-bath attached to a reflux condenser and collect the dis- 

 tillate. 



