942 EXPERIMENTAL INFECTION AND IMMUNITY 



2. Evaporate the distillate to dryness and for each 10 c.c. of the distillate add 

 5 c.c. of water. Neutralize with ^ hydrochloric acid. 



3. Inject 5 c.c. intraperitoneally into young guinea-pigs. Inject 1 or 2 c.c. intra- 

 venously. 



(a) Do anaphylactic symptoms develop? 



(b) Are the anatomic changes in the lungs similar to those observed 

 in serum anaphylaxis? 



(c) Discuss the prevailing views of the nature of anaphylatoxins. 



(d) Discuss the prevailing views of the anaphylaxis antibody, toxo- 

 gen, or anaphylactin. 



EXERCISE 56. ANAPHYLAXIS (Continued) 

 EXPERIMENT 109. ANAPHYLAXIS IN THE DOG 



1. Five weeks after sensitization anesthetize a dog with ether and connect the 

 carotid or femoral artery with a mercurial manometer and arrange for a kymographic 

 tracing. 



2. After recording the normal blood-pressure tracing, inject 5 c.c. of homologous 

 serum (horse) into a jugular vein. 



3. Record the blood-pressure. 



4. Study the coagulation time of the blood. 



5. Autopsy. 



(a) Describe the blood-pressure changes. To what may they be 

 ascribed? 



(b) Is blood coagulation delayed? Give a reason for this change. 



(c) Do anatomic changes occur in anaphylaxis of the dog? 



EXERCISE 57, ANAPHYLAXIS (Continued) 

 EXPERIMENT 110. PASSIVE ANAPHYLAXIS 



1. Secure 1 c.c. of serum from a rabbit sensitized to horse serum five weeks previ- 

 ously and inject 0.5 c.c. intraperitoneally into each of two guinea-pigs. 



2. Twenty-four and forty-eight hours later inject both animals intravenously 

 with 0.2 c.c. of horse serum. 



(a) Are anaphylactic symptoms in evidence? 



(b) Discuss the nature of passive anaphylaxis. 



(c) If allowed to live, would these animals become sensitized to rab- 

 bit serum? 



EXERCISE 58. ANAPHYLAXIS (Continued) 

 EXPERIMENT 111. ANTI-ANAPHYLAXIS 



1. Eight days after sensitizing a guinea-pig with horse serum, inject 1 c.c. of 

 horse serum subcutaneously. 



