ANAPHYLAXIS 943 



2. Fifteen days after sensitizing a guinea-pig with horse serum, inject 2 c.c. of 

 serum into the rectum. 



3. Fifteen days after sensitizing a guinea-pig with horse serum, inject 0.0001 c.c. 

 serum subcutaneously every fifteen minutes for six doses. 



4. On the sixteenth day after sensitizing, inject a guinea-pig with 1 c.c. of horse 

 serum. If this animal develops anaphylactic shock, inject the other three guinea- 

 pigs in a similar manner. 



(a) Do anaphylactic symptoms develop? If not, why not? 



(b) Discuss the importance of quantitative factors in producing 

 anti-anaphylaxis. 



(c) Discuss the importance of anaphylaxis and anti-anaphylaxis 

 in serum therapy. 



(d) What method is generally employed in the effort to induce an 

 anti-anaphylactic state in serum therapy? 



(e) What drug has been found experimentally of value in ameliorat- 

 ing or preventing the pulmonary changes of anaphylaxis? 



(f) Do anesthetics prevent anaphylaxis? 



(g) Under what conditions would you expect anaphylaxis in persons? 



EXERCISE 59. ANAPHYLAXIS (Continued) 

 EXPERIMENT 112. LOCAL ANAPHYLACTIC REACTIONS 



1. Shave the abdomen of the rabbit sensitized to horse serum five weeks after 

 the tune of sensitization. 



2. Make two superficial abrasions over the upper portion of the abdomen; into 

 one rub horse serum, and in the other, normal salt solution. 



(a) Does a local reaction occur? If so, describe the lesion. 



3. Then inject 0.01 c.c. of serum subcutaneously. If acute anaphylactic death 

 does not follow, observe the animal during the next twenty-four hours. 



(a) Does a local Arthus reaction follow this injection of serum? If 

 so, describe the reaction. 



(b) Explain the mechanism of a local anaphylactic reaction. 



(c) Are the tuberculin, luetin, and mallein reactions anaphylactic? 

 Explain their mechanism and discuss their practical value in diagnosis. 



4. Inject 1 c.c. horse serum intravenously. If anaphylaxis occurs, record the 

 symptoms. Autopsy with particular attention to the heart. 



