225 



1.5 g. sodium chloride. 



1.5 g. neutral potassium phosphate. 



600 cc. distilled water. 



SOLUTION LACKING POTASSIUM. 

 g. calcium nitrate. 

 1.5 g. magnesium sulphate. 

 1.5 g. sodium chloride. 



. i . 5 g. neutral sodium phosphate, or calcium phosphate in excess^ 

 600 cc. distilled water. 



SOLUTION LACKING MAGNESIUM. 

 , 6 g. calcium nitrate. 

 1-5 g. potassium nitrate. 

 1.5 g. neutral potassium phosphate. 



1.2 g. potassium sulphate or an excess of calcium phosphate. 

 600 cc. distilled water. 



SOLUTION LACKING NITRATES. ' 

 l/f.5.g. neutral potassium phosphate. 

 \/ i-5 g. magnesium phosphate". 

 1.5 g. potassium chloride. 

 600 cc. distilled water. 



SOLUTION LACKING PHOSPHATES. 

 0.5 g. potassium nitrate. 

 I g. calcium nitrate, 

 v 0.5 g. magnesium nitrate. 



0.5 g. neutral potassium sulphate. 

 1 ,000 cc. distilled water. 

 ^i^This solution is to be used without the addition of more water. 



' * 4lk. 



K drop of solution of iron chloride should be added to the 

 culture jar every time it is filled or refilled. The chemicals used 

 should be absolutely pure. A daily aeration of the solutions in 

 the jars by means of a blower attajj|ed to a water tap, or an as- 

 pirator will be an advantage (Figs. 92, and 1 11). The solutions 

 in the stock bottles should be well shaken up before the necessary 



16 



