810 
example, furnished from a patient sufferin 
from : chronic cerebral affection. : 
(a) The entire blood employed amounted to 
6735 grs.; it had been drawn 24 hours, and 
had formed a tolerably firm but flat coagulum, 
which weighed 3560 grs. The serum that had 
weighed 3175 grs., and had a sp. gr. 
of 1029.3, es a 
The proportions of clot and serum were 
therefore as follows, in 1000 parts. 
6735 : 1000 : : 3560: « (=528.6)coagulum. 
6735 : 1000 : : 3175 : «(= 471.4) serum. 
The analysis divides itself into two portions, 
that of the serum, and that of the clot. 
Analysis of the serum. 
(6) 200 grs. of serum were dried at a tem- 
perature of 212°. The residue amounted to 
18.69grs. 
200 : 1000 :: 18.69 : « (= 93.45) solids 
in serum. 
Therefore 1000 — 93.45 = 906.55, water 
in the serum. 
(c) The residue was incinerated over the cir- 
cular wicked spirit lamp, and amounted to 
1.46 grs. 
200 : 1000 :: 
matters. 
(d) 500 grains of serum were dried in a pla- 
tinum capsule, the dry mass carefully detached, 
pulverized, and digested in boiling ether, which 
was decanted and renewed three or four times. 
The ethereal solutions evaporated left 0.35 grs. 
of fatty matter; of this cold alcohol dissolved 
0.12 oily fat, leaving 0.23 of crystalline fats. 
1000 parts of serum therefore contain 0.24 
oily fat, and 0.46 crystalline fats. 
(e) The undissolved residue was heated to 
expel adhering ether and treated with boiling 
water. It was thrown on a filter and washed 
repeatedly, until nitrate of silver produced an 
insignificant cloud when applied to a few 
drops of the washings. The filter and its con- 
tents were dried; when weighed the albumen 
amounted to 41.73 grs. 
10 grs. when incinerated yielded 0.19 grs. of 
ash ; 10 : 41.73::0.19 : e(= 0.79287.) 
41.73 — 0.793 = 40.937; 40.937 x 2 = 
81.974 albumen. 
(f) The filtered liquid evaporated weighed 
4.66 grs. It was digested with alcohol, which 
was renewed as long as any thing was dis- 
solved. The alcoholic solution evaporated 
amounted to 3.32grs. (If urea, sugar, or bile 
be present, they will be contained in this extract 
and must be sought for in the usual manner.) 
4.66 — 3.32 = 1.34 grs. watery extract, 
1.34 x 2 = 2.68, 1gr. of watery 
extract incinerated left 0.82 grs. of 
salts, and 1. : 2.68: 0.82 : t = 2.197, 
and 2.68 — 2.197 = 0.483 watery 
extract 1000. 
) 1.4 grs. of alcoholic extract, incinerated, 
left 1.00 grs. of saline matter. 
Now 1.4:1 :: 6.64 : x (= 4.74) saline 
matters, 
And 6.64 — 4.74 = 1.90 alcoholic extract 
per 1000, 
1.46 : « (= 7.3) saline 
ORGANIC ANALYSIS. 
The serum therefore consists of } 
Water, 25.00. chan ones 906.550 . 
Fixed saltssicee cath. d3% 7.300 
Albumen ...........4.- 81.974 
Alcoholic extract .....8.. 1.900 
Watery extract .......2.. 0.483 
Fats, oily Core erersces “* 0.240 
»» crystalline ,......... 0.460 
998.907 
Analysis of the clot. 4 
(hk) 1000 grs. shred finely with a sharp knife 
were tied in a piece of calico and washed till 
colourless under a gentle stream of water. 
The fibrin that remained carefully dried at 212° 
weighed 5.67 grs. - 
Ether dissolved from this 0.07 grs. of oily fat. — 
The pure fibrin therefore amounted to 5.60 grs. 
1000 : 528.6 : : 5.6 : x (= 2.962) fibrin. 
1000 : 528.6 by (a) : : 0.07: (= 0.04) © 
Sat from firm. q 
(i) 500 grs. of the coagulum completely dried 
at 212° left a residue of 143.6 grs. 
500 : 528.6 : : 143.6 : t (= 151.81) solids 
in the clot. 
k) 528.6 — 151.8 = 376.8 water in the clot. 
upposing the water entirely due to the 
serum with which the clot is penetrated, 
should find it contain— 
Since by (6) 906.55 : 376.8:: 93.45 : & 
(= 38.86) solids of the serum retained 
by the clot, and a 
376.8 + 38.86 = 415.66 weight of tl 
serum retained. y 
But 415.66 + 471.4 by (a) = 887.06 tot 
serum in 1000 parts of blood. 
(2) 20 grs. of the dried matter of the coagu- 
lum ignited lefta red ash, weighing 0.52 grs. 
20 : 151.81 :: 0.52: 2 (= 3.947) salts it 
the clot. 
But 1000:471.4 2: 7.3 by (c) : 2 (= 3.4 
salts in the exuded serum. 
3.947 + 3.441 = 7.388 salts in 1000 
of blood. ». 
(m) Since the coagulum contains 2.962 fibrin, 
And the serum retained ...... 38.860 solic 
The sum of the two = 41,822 
And by deducting this sum from the 
solids contained in the clot we find 
151.81 — 41.822 = 109.988 red p 
in 1000 of blood. 
(n) In order therefore to deduce the comp 
sition of 1000 parts of blood, we have only 
calculate the following proportions from + 
knowledge of the corepostion ae the serum. 
1000 : 887.06 :: 906.55: 2 (= &@ 
holic extract. 
1000 : 887.06 :: 0.483 
watery extract. 
1000 : 887.06 : : 0.24 : x (= 0.213) of 
To this we must add 0.04 from the fi 
making the total oily fat = 0.253. 
: a (= 0. 
