CONTENTS. 



INTRODUCTION. 



PAGES 



"Orane vivum ex vivo" The overthrow of the doctrine of 

 spontaneous generation Earlier bacteriological studies 

 The birth of modern bacteriology 17-30 



CHAPTER I. 



Definition of bacteria Difference between parasites and 

 saprophytes Their place in nature Bacterial enzymes 

 Products of bacteria Nutrition of bacteria Their relation 

 to oxygen Influence of temperature upon their growth 

 Cheinotaxis 31-48 



CHAPTER II. 



Morphology of bacteria Chemical composition of bacteria 

 Classification of bacteria into families and genera Group- 

 ing Mode of multiplication Spore-formation Motility 

 The thermal death-point of bacteria 49-68 



CHAPTER III. 



Principles of sterilization by heat Methods employed Dis- 

 continued sterilization Fractional sterilization Appa- 

 ratus employed Sterilization by hot air Sterilization 

 under pressure Chemical disinfection and sterilization 

 Mode of action of disinfectants Practical disinfection . . GO-!Mi 



CHAPTER IV. 



Principles involved in the methods of isolation of bacteria 

 in pure culture by tin- plate method of Koch Materials 

 employed 97-103 



CHAPTER V. 



Preparation of nutrient media Bouillon, gelatin, agar-atrar. 



potato, blood-serum, etc 104-137 



vii 



