314 BACTERIOLOGY. 



position ; lot that in the other remain fluid. On the 

 surface of the former pour 0.5 c.c. of the pyocyaneus 

 filtrate, and mark the point of contact between the 

 gelatin and filtrate. To the other tube add a similar 

 amount of the filtrate, mix thoroughly, and solidify in 

 a glass of cold water. 



At the end of eighteen to twenty hours note result. 

 Is it possible to solidify again the gelatin through which 

 the filtrate was mixed, by placing the tube in cold water? 



Do the activities of this enzyme suggest those of any 

 of the enzymes encountered in the animal body ? 

 Which? and Why? 



INOCULATION INTO ANIMALS. As a rule, cultures 

 of this organism obtained directly from the discharges 

 of the wound are capable, when introduced into ani- 

 mals, of producing diseased conditions ; but cultures 

 kept on artificial media for a long time may in part, or 

 completely, lose this power. 



When guinea-pigs or rabbits are inoculated subcuta- 

 neously with 1 c.c. of virulent fluid cultures of this 

 organism, death usually results in from eighteen to 

 thirty-six hours. At the seat of inoculation there are 

 found an extensive purulent infiltration of the tissues 

 and a marked zone of inflammatory oedema. 



When introduced directly into the peritoneal cavity 

 the results are also fatal, and at autopsy a genuine 

 fibrinous peritonitis is found. There is usually an ac- 

 cumulation of serum in both the peritoneal and pleural 

 cavities. At autopsies after both methods of inocula- 

 tion the organisms will be found in pure cultures in 

 the blood and internal viscera. 



When animals are inoculated with small doses (less 

 than 1 c.c. of a bouillon culture) of this organism death 



