5 1 4 BA CTERIOLOG Y. 



appear at the periphery of the colony. At the ex- 

 treme periphery of the colonies it is sometimes possible 

 to trace single bundles of these threads for long dis- 

 tances across the surface of the agar-agar. The colony 

 itself is not circumscribed in appearance, but is more 

 or less irregularly fringed or ragged, or scalloped. To 

 the naked eye they look very much like minute pellicles 

 of raw cotton that have been pressed into the surface 

 of the agar-agar. 



As the colonies continue to grow they become more 

 and more dense and opaque, and granular and rough on 

 the surface. When touched with a sterilized needle 

 one experiences a sensation that suggests somewhat 

 their matted structure. They are never moist or 

 creamy. The bit that is taken up with the needle is 

 always more or less ragged. 



GELATIN. The colonies on gelatin at the earliest 

 stages also present the same wavy appearance ; but this 

 characteristic soon becomes in part destroyed by the 

 liquefaction of the gelatin which is produced by the 

 growing organisms. This allows them to sink to the 

 bottom of the fluid, where they lie as an irregular mass. 

 Through the fluid portion of the gelatin may be seen 

 small clumps of growing bacteria, which look very much 

 like bits of cotton- wool. 



BOUILLON. In bouillon the growth is characterized 

 by the formation of flaky masses, which also have very 

 much the appearance of bits of raw cotton. Micro- 

 scopic examination of one of* these flakes reveals the 

 twisted and plaited arrangement of the long threads. 



POTATO. On this medium it develops rapidly as a 

 dull, dry, granular, whitish mass, which is more or less 

 limited to the point of inoculation. On potato, at the 



