BACTERIUM ANTHRACIS. 521 



Pasteur method : (1) That the immunization can be car- 

 ried out without losing any of the animals ; (2) that it 

 can be completed in one day ; (3) that stronger and more 

 active cultures can be employed and therefore a more 

 durable immunity obtained; and (4) that the serum alone 

 can be employed as a curative agent. 



Schlemmer employed the Sobernheim method on 39 

 oxen in which the results were not uniformly satis- 

 factory, as one of the animals became ill and most of 

 them showed febrile reactions. Schlemmer attributes 

 the unfavorable results to the small dose of immune 

 serum employed, namely, 10 cubic centimetres, and 

 believes that for large animals larger doses of the 

 immune serum should be used. 



EXPERIMENTS* 



Prepare three cultures of bacterium anthracis one 

 upon gelatin, one upon agar-agar, and one upon potato. 

 Allow the gelatin culture to remain at the ordinary 

 temperature of the room, place the agar-agar culture 

 in the incubator, and the potato culture at a temper- 

 ature not above 18 to 20 C. Prepare cover-slips 

 from each from day to day. What differences are 

 observed ? 



Prepare two potato cultures of bacterium anthra- 

 cis. Place one in the incubator and maintain the 

 other at a temperature of from 18 to 20 C. Ex- 

 amine them each day. Do they develop in the same 

 way? 



From a fresh culture of bacterium anthracis, in which 

 spore-formation is not yet begun (which is the surest 



