670 APPENDIX. 



handles to be about 3 mm. in thickness and from 15 

 to 17 cm. long. 



STAINING- AND MOUNTING-REAGENTS. 



200 c.c. of saturated alcoholic solution of fuchsin. 



200 c.c. of saturated alcoholic solution of gentian- 

 violet. 



200 c.c. of saturated alcoholic solution of methylene- 

 blue. 



200 grammes of pure aniline. 



200 grammes of C. P. carbolic acid. 



500 grammes of C. P. nitric acid. 



500 grammes of C. P. sulphuric acid. 



200 grammes of C. P. glacial acetic acid. 



1 litre of ordinary 93-95 per cent, alcohol. 



1 litre of absolute alcohol. 

 500 grammes of ether. 

 500 grammes of pure xylol. 



50 grammes of Canada balsam dissolved in xylol. 

 100 grammes of Schering's celloidin. 

 10 grammes of iodine and 30 grammes of potassium 

 iodide in substance. 



100 grammes of tannic acid. 

 100 grammes of ferrous sulphate. 

 Distilled water. 



FOR NUTRIENT MEDIA. 



\ pound of Liebig's or Armour's beef-extract. 

 250 grammes of Witte's or Sargent's peptone. 



2 kilogrammes of gold-label gelatin (Hesteberg's). 

 100 grammes of agar-agar in substance. 



200 grammes of sodium chloride (ordinary table-salt). 

 500 grammes of pure glycerin. 



