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one had a strong irritability at the end of forty-one hours after 

 the death of the animal ; 2, That its rigidity gave way to putre- 

 faction only at the eightieth hour ; 3, That it was in complete 

 putrefaction only at the ninety-fourth hour. The other limb 

 was in full rigidity at the fifth hour after the death of the 

 animal ; its rigidity gave way to putrefaction at the forty- 

 eighth hour ; and it was in complete putrefaction at the seven- 

 tieth hour. 



From all the experiments above related, it appears that life 

 may be reproduced or maintained in muscles and nerves by 

 mere injections of blood. I have found, also, that life may be 

 reproduced by the same means in the spinal cord and in the 

 brain. I will publish these facts in another article. 



It is nearly indifferent in these experiments whether we use 

 venous or arterial blood ; but it is absolutely necessary to employ 

 red blood, i. e. oxygenated blood. 



I have tried, sometimes, arterial blood, rendered black by 

 the substitution of nitrogen or hydrogen for a great part of its 

 oxygen, and I have found that such blood was unable to repro- 

 duce the vital properties of nerves and muscles. 



Oxygen is necessary, either because it prevents the blood-glo- 

 bules from being altered, or because it acts directly on muscles, as 

 Gustavus Liebig has found it does on their external surface, 

 when exposed to air. I believe it is necessary for both these rea- 

 sons. 



I cannot say how long after the beginning of cadaveric 

 rigidity in a muscle, oxygenated blood can reproduce local life. 

 In the second of the two decapitated men, on whom I ex- 

 perimented, rigidity had existed at least five hours before the 

 injection was begun. I believe that the stronger the animal is, 

 the more easy it is to reproduce local life in rigid limbs, by 

 injection of blood. In limbs of weak rabbits, I have found it 

 impossible, two hours after the beginning of cadaveric rigidity, 

 to reproduce local life. In a very strong dog I have reproduced 

 muscular irritability four hours after rigidity had been fully 

 developed. 



Ten, twelve, or fourteen hours after rigidity had taken place, 

 in human limbs, I have tried in vain to re-establish local life. 



I have ascertained that pure serum of blood, or milk, or albu- 



