lOG CIRCULATING FLUIDS: 



weight of serum^ but the difference is by no means so striking 

 as it would have been if Ascherson's theory were correct. 



Hiinefeld i has obsei'ved a similar appearance on treating 

 the blood-coi'puscles of the frog with putrid serum, in which 

 granules were present. The granules seemed to form a sort 

 of girdle round the corpuscle, and he conceives that they 

 penetrated into minute depressions upon the surface of the 

 capsule. If this statement be correct, it is strongly opposed 

 to the observations of Ascherson and Wagner respecting the 

 lubricity and evenness of the blood-corpuscles. 



On mixing the blood of a caq) with a solution of sugar, 

 and on the cautious addition of water, T observed that the 

 blood-corpuscles assumed a stellar appearance. 



On treating frog's blood with bilin, an agent which usually 

 dissolves the corpuscles, I observed that some of them resisted 

 this action for a considerable period, and ultimately assumed 

 a pyriform appearance, while others became narrowed at the 

 centre, and extended at both extremities. Others, again, 

 seemed to undergo an internal change, and appeared as if 

 their inner surface were studded with minute vesicles. 



Hiinefeld made a similar observation on treating frog's 

 blood with carbonate of ammonia.'^ 



The same chemist observed a remarkable peculiarity in the 

 corpuscles of human blood, on the addition of sulphate of 

 quinine. In the course of a few minutes they assumed an 

 irregular, angular form, and appeared as if their sides were 

 drawn together. 



Schultz^ has made the following important microscopic ob- 

 servation. On examining the blood-corpuscles of a salaman- 

 der which had been suffocated in carbonic acid gas, they were 

 found to be of a darker colour than usual ; the darkness was 

 particularly marked on some spots, so that they exhibited a 

 sort of chequered appearance. 



On shaking the blood with oxygen gas, the corpuscles be- 

 came brighter and more transparent. 



' Der Chemismus in der thicrischen Organisation, p. 101. 



■ L. c, p. 106. 



^ System der Cirkulatioii, p. 27. 



