170 CIRCULATING FLUIDS: 



In the separation of the haematoglobulin from the fibrin, ac- 

 cording to this method, about the seventieth part of the clot 

 is lost in the water. The solution of the colouring matter is 

 heated until the coagulation of the hcematoglobulin is ef- 

 fected, which is then separated and freed from moisture by 

 presstire. 



The fluids are then evaporated to dryness. We have now 

 four subdivisions : 



a. The fibrin which still contains fat and cruorin.i 



b. Albumen with cruorin, salts, and extractive matter. 



c. Haematoglobulin with fat, extractive matter, and salts of 

 iron and the earths. 



d. The evaporated fluid separated from the haematoglobulin, 

 containing salts and osmazome. 



These four portions are dried, weighed, put into glass flasks, 

 and submitted for some minutes to the action of alcohol of 

 •800 — -820, at a temperature of 86°; they are then filtered, 

 and the spirituous solutions united and evaporated. The re- 

 sidue, consisting of extractive matters and salts, must be in- 

 cinerated, by which means the quantity of extractive matter is 

 determined. 



The four portions must now be treated with boiling water, 

 by which cruorin and certain salts are removed. 



The portions a and d are now combined, and the three are 

 treated with boiling alcohol of -800 for the purpose of ex- 

 tracting the fat. The filtered solutions are united and evapo- 

 rated, and the cholesterin separated by crystallization from the 

 fats which contain phosphorus. The mixed portion of a and d 

 contains tolerably pure albumen ; it is dried, weighed, and in- 

 cinerated, and the ash is preserved. 



The second portion contains fibrin; this likewise is incine- 

 rated, and the ash added to the former. 



Lastly, the haematoglobulin is dried, weighed, and incine- 

 rated. The collected ashes are analysed with regard to the pro- 

 portions of peroxide of iron, phosphates of lime and mag- 

 nesia, &c. 



' Denis applies the term cruorin to a substance obtained by boiling fibrin and 

 albumen in water. It is soluble in water, insoluble in alcohol and ether, of an agree- 

 able taste, and precipitable by tannic acid. It ai)pears to be produced ])y the action 

 of the boiling water on fibrin previously affected by long contact with water. 



