188 CIRCULATING FLUIDS : 



not a bitter taste. I am not aware that bilin or bilifellinic acid^ 

 has ever been observed in the blood, and I hardly believe that 

 they will be found, owing either to their not being taken up 

 by the blood at all, or else to their speedy ebmination by the 

 urine. A large quantity of bilin would have a very dangerous 

 effect upon the blood, since (as we have already shown) it dis- 

 solves the blood-corpuscles. I treated 500 grains of blood with 

 half a grain of inspissated ox-bile, and then precipitated the 

 protein-compounds with spirit, evaporated the fluid, and treated 

 the residue with anhvdrous alcohol. It is clear that the bile 

 must be contained in this residue. After the evaporation of the 

 alcohol, there remained a rather dark-coloured extract, haA^ng 

 a bitter bile-like taste, and which, when dissolved in water, and 

 nitric acid was added, manifested a slight green tinge. If, there- 

 fore, the bilin should constitute one-thousandth part of the blood, 

 it would be easily detectible. 



If the analysis of the fats and of the extractive matters is to 

 be thoroughly carried out, (as in many cases it certainly ought 

 to be,) much larger quantities of blood must be taken than I 

 have made use of. 



The various fats, however, as well as the difterent extractive 

 matters, are at present too little known to enable us to attempt 

 exact, or even approximating quantitative analyses. 



4. Fats. Boudet^ has analysed the fats which are taken up 

 by alcohol from dried blood, after all substances that could be 

 extracted by water have been removed. The alcoholic solution 

 deposits serolin on cooling, which must be separated, and the 

 alcohol evaporated. There remains as a residue a mixture of 

 several fats, which were separated by Boudet in the following 

 manner. Cold alcohol of 'SSS leaves undissolved a crystaUine 

 fat which contains phosphorus, and is apparently similar to the 

 brain-fat, denominated cerebrot by Couerbe. Cholesterin is 

 deposited by the spontaneous evaporation of the cold alcohoUc 

 solution ; and on further evaporation, (after the removal of the 

 cholesterin,) there is left a mixture of oleic and margaric acids, 



' [Enderlin states that he has detected minute quantities of choleate of soda (pure 

 bile, according to Demarfay's theory) on three occasions, in the blood of calves and 

 oxen. (Annales der Chemie und Pharmacie, April 1844.)] 



^ Annal. dc Chim. et de Phys., vol. 52, p. 337. 



