10 



field, in several of the plugs from each "donor" pond, 



Thirty plugs were taken from each "donor" pond, and placed in 

 plastic bags for transport to the transplant sites. Fifteen 

 plugs were placed along transects in each of the four transplant 

 ponds. The ends of these transects are marked with steel posts 

 that were painted orange. The plugs were placed in the 

 transplant ponds as follows: 



POND TRANSECT LOCATION; TRANSECT 

 DIRECTION READ BEARING 



PLUG PLACEMENT 



A Across west edge of 3 20 

 pond; S->N 



B Along southeastern 76° 

 edge of pond; W->E 



1.6, 3.1, 6.0, and 8.4 

 m, then every 2 m from 

 10.0 to 30.0 m. 



Every 2 m from 2.0-10.0 

 m and 20.0-24.0 m; every 

 1 m from 12.0-18.0 m. 



D 



Along northeastern 177 e 

 edge of pond; N->S 



Along northeastern 14 l c 

 edge of pond; N->S 



Every 2 m, from 2.0- 

 30.0 m. 



Every 2 m, from 2.0- 

 30.0 m. 



The transects in ponds A and B were not under water on the 

 transplant date (27 SEP 1989) . Soil plugs were placed in holes 

 dug in the pond sediments with the tin can. The transects in 

 ponds C and D were placed in water approximately 30 cm and 25 cm 

 deep, respectively (28 SEP 1989) ; the plugs were placed on the 

 pond bottoms. 



The transplant transects should be re-visited in mid-July, 1990, 

 to assess success. Ponds where the plugs were placed under water 

 (C and D) should be thoroughly surveyed; some H. aquatilis seeds 

 were observed to float when the plugs were put in the water, and 

 those seeds may have become established away from the transect 

 lines. 



DISCUSSION: Recent detailed studies of the seed germination 

 ecology of H. aquatilis indicate that the species is not capable 

 of germinating under water (Lesica 1990) . In these transplant 

 experiments, two of the four ponds receiving soil plugs still 

 contained water on the transplant date (C and D) ; it is unlikely 

 that these ponds dried out much further prior to freezing. The 

 transects in the other ponds (A and B) were not under water on 

 the transfer date. Thus, these different transplanting 

 treatments may provide a field test of the observed laboratory 

 germination of H. aquatilis seeds. Also, 1990 and 1991 

 observations of the ponds that did not dry in 1989 may 

 substantiate the hypothesis that, in ponds that do not dry by the 



