152 ARTIFICIAL PARTHENOGENESIS AND FERTILIZATION 
6. The inhibitive effect of KCN and lack of oxygen upon the 
activation of the unfertilized egg by bases suggested an investi- 
gation of the influence of the various bases upon the rate of 
oxidations in the unfertilized egg, to find out whether the weaker 
bases raised the rate of oxidations more than the stronger ones.! 
The experiments were carried out on the unfertilized egg 
of S. purpuratus. The oxygen content was determined ac- 
cording to Winkler’s method. The experiments were made 
in a half grammolecular mixture of NaCl+KCl+CaCl, in that 
proportion in which these three salts are contained in the sea- 
water. 
We first give the results of a series of experiments in which 
the relative influence of various bases was compared. The 
TABLE XXVI1 
Se mem. ot | Acpeleration 
- Nature of the Solution Oxygen : . 
na constimea | O=dations, 
I Neutral eines. cin cte oer eek eae i= 0228 
**|\50 c.e. neutral+0.3 ¢.c. N/10 NaOH.. 0.40 1.43 
Il (Neutral By Pn Sun GR os oe RGR 0.15 
“1150 e.e. neutral+0.3 e.e. pie tetra- 
ethylammoniumhydroxide. . so 3 0.22 1.50 
ry. .|{Neutral..........0... ee : a 0.30 
“1150 e.c. neutral+0.3 c.c. N/10 NH,OH. 0.81 2.70 
IV pNewtral.? acre hota ee eee ee | 0.40 
“1150 ¢.c. neutral+0.3 ec. N/10 tr-| 
Me ty AMIN eee ener eee [agai iQ 3.00 
V {Neutral EE eRe dares ies ne ety 0.25 
| 50 e.e. neutral+0.3 e.c. N/10 methyl-) 
AMM eas nS ee Cae ae 1.18 4.70 
VI NiaNte Utils ese aan ate eae hei, nee ees 0.28 
**|\50 e.c. neutral+0.3 ¢.c. N/10 ethyl-| 
AMINED. Se: BENS eR Ree ee ee 1.35 4.80 
VU {Neutral Bi Wale. che Getica's lols foils Goyieit Memel outa -otiche eriep ewes 0.32 
“1150 c.c. neutral+0.3 c.c. N/10 butyl- 
AMIN Cli 2 9. Seen ae esesy ees ce ona [A238 3.80 
VIII Neutral eee eee eae |= = 10)522 
“1150 e.c. neutral+0.3 e.c. N/10 benzyl-, 
DINING oes ees oe ee oe 1.30 5.90 
1 Loeb and Wasteneys, Jour. Biol. Chem., XIV, 355, 1913. 
