SARCINA FLAVA. 159 



in Wiirzburg. In cultures it remained constant for a year, the origi- 

 nal active liquefaction only being somewhat lessened. The three 

 following are subspecies or varieties: 



Sarcina livido=Iutescens (Stubenrath). 



Like Sara equi, but young potato cultures for ten days and more 

 are gray to reddish-gray; after twenty days they become brownish- 

 yellow in the center, and after a month throughout the entire culture. 

 The constancy of this characteristic was observed for a year. In a 

 case of enteritis it was grown abundantly from the stool by Dr. 

 Stubenrath. 



Sarcina canescens (Stubenrath). 



Differentiated from Sarc. equi only by constant gray color and 

 somewhat coarser granulation (larger bales of packets) upon all 

 nutrient media (5, vin). 



Sarcina variabilis (Stubenrath). 



This form, isolated from gastric contents, appears to us to be very 

 interesting. It is differentiated from the Sarc. equi only by more 

 marked liquefaction of gelatin and by the property of furnishing on 

 the various nutrient media sometimes yellowish-gray, sometimes pure gray 

 colonies. Upon plates one often obtains gray and yellowish colonies 

 side by side, but this is alike repeated whether one inoculates from 

 gray or yellowish colonies. 



Sarcina flava (de Bary, emend. Lehmann and 

 Stubenrath). 



(Plate 3.) 



Upon all nutrient media it is habitually very similar to the Sarc. 

 lutea, being yellow to greenish-yellow. The principal difference lies 

 in the very finely granular gelatin plate colonies when magnified sixty 

 times. When magnified one thousand times, this fine granulation is 

 seen to depend upon very small bales and heaps of packets. l We have 

 observed one form that is more luxuriant and distinctly liquefying, and 

 one that is more delicate, leaving the gelatin still solid after weeks, 

 growing feebly upon all nutrient media. It has been repeatedly culti- 

 vated from gastric contents. 



1 The Sarc. flava, obtained from Krai, Dr. Stubenrath found to 

 form upon all fluid and solid nutrient media, usually only bunches of 

 cocci, rarely tetrads, and never true bales of packets. 



