MICROCOCCUS LUTE US. 177 



Gelatin Stab. — Stab : Until liquefaction begins it is 

 granular. After two days liquefaction begins with a plate- 

 shaped depression, which later becomes cylindric. The 

 contents of the funnel are cloudy, greenish or yellowish-gray 

 (6, i). 



Agar Plate. — Both when of natural size and when 

 magnified fifty times, the colonies are like those in the 

 gelatin plate, only the granulation is finer. Sometimes 

 there are found thin, pale yellow, transparent deep colo- 

 nies, as much as 2 mm. broad, with a coarsely granular 

 or morulated structure. 



Agar Stab. — Stab : Granular, yellow. Surface-growth : 

 Lemon-yellow, shining, roundish, with wavy border, some- 

 what elevated. 



Agar Streak. — Corresponding to the stab. Water of 

 condensation clear, sediment yellowish. 



Bouillon Culture. — Remains clear. The yellowish 

 precipitate is closely packed, rising up tenaciously only 

 with energetic shaking, and afterward becoming homoge- 

 neously divided. 



Milk Culture. — After twenty days it is half coagulated. 

 Acid reaction. 



Potato Culture. — Lemon-yellow to yellowish-green, 

 thin, faintly shining, with a wavy, irregular border, and 

 almost no elevation whatever. Sharply outlined from the 

 surroundings. 



Related Varieties. 



We consider this form identical with sarcina lutea — only 

 our form produces no sarcina groups, either upon solid 

 nutrient media, or in bouillon, or even in hay infusion. 

 Sarcina lutea would be its "sarcina form." 



The Streptococcus liquefaciens and Pediococcus flavus, 1 obtained 

 from Krai, are identical. The Strept. liquefaciens only produces a 

 cloudiness of bouillon and of the funnel of liquefied gelatin, and in old 

 agar streaks has a brownish-yellow shade — variations which are con- 

 stantly observed in Micr. pyogenes a aureus. From the description the 

 Micr. galbanatus Zimmermann is also identical, and, as we saw 

 subsequently, was found by Zimmermann to be identical with the 



1 Recently we have discovered beautiful packets of sarcina from old 

 hay infusion cultures of Pedioc. flavus. These were not so evident in 

 Strept. liquefaciens. 

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