282 IMPORTANT VARIETIES OF FISSION-FUNGI. 



Gelatin Plate. — (a) Natural size. Deep : Roundish to 

 whetstone-shaped, yellowish-white to greenish-yellow. 

 Sometimes also there is a roundish, spreading, transparent, 

 greenish-yellow extension with the original colony in the 

 center. Superficial: At first roundish, uneven, delicately 

 spreading, but immediately saucer-shaped liquefaction 

 occurs. There is often a lighter peripheral zone. The 

 liquefied material is cloudy, and gray to greenish-gray. 

 The original colony appears as a crumbly mass at the 

 center (24, v). There is intense fluorescence about the 

 colony. 



(6) Magnified fifty times: Both the superficial and deep 

 colonies are yellowish, roundish, with smooth border, and 

 delicately punctate at first. After twelve to twenty-four 

 hours the superficial colonies have a transparent, ragged 

 border (like Bact. coli), and are also sometimes beset with 

 little hairs or fringes. Then immediately begins the de- 

 pression of the colony (24, in). The color becomes brown- 

 ish, the irregular form and ring of hairs are partly lost, 

 the contents of the liquefied area are uniformly crumbly. 

 The periphery and the structure of the colony appear with 

 the greatest variations, sometimes ragged, sometimes gran- 

 ular, sometimes punctate, sometimes lighter, sometimes 

 darker, until the colony falls entirely apart. The middle 

 portion of the colony usually survives and is darker in 

 color (24, iv). (Compare also 25, v and x.) 



Gelatin Stab. — Liquefaction begins very early, is at 

 first cup-shaped, later cylindric, and more rarely is shaped 

 like a pointed funnel. The liquefied material is slightly 

 cloudy, with a greenish-yellow to bluish-green fluorescence. 

 There is gradual liquefaction along the stab canal, the con- 

 tents being yellowish and crumbly (24, i) . 



Agar Plate. — (a) Natural size. Deep: Roundish to 

 whetstone-shaped, non-characteristic, yellowish. Super- 

 ficial: Roundish, smooth-bordered, with a moist luster, 

 greenish-white to yellowish. There is intense greenish-yel- 

 low fluorescence of the surrounding medium (24, vi). 



(b) Magnified fifty times. Deep : Roundish to whetstone- 

 shaped, with a border partly smooth, partly delicately 

 wavy, delicately punctate or granular (like Bact. coli), 

 light yellow to greenish -yellow. Superficial: Usually 



