302 IMPORTANT VARIETIES OF FISSION- FUNGI. 



Bouillon Culture. — No pellicle, little turbidity, very 

 little sediment, which is very slightly coherent. 



Milk Culture. — No coagulation, amphoteric or feebly 

 alkaline reaction. 



Potato Culture. — No perceptible growth. 



Chemical Activities. — No formation of pigment or 

 odoriferous substances. Our culture produces no H 2 S or 

 indol. Petri and Maassen observed vigorous production 

 of H 2 S. A little acid is formed from grape-sugar and 

 gelatin is very slowly liquefied. 



Distribution. — 



(a) Outside the body : Isolated repeatedly by Koch and 

 others from canal-water and putrid mixtures (putrid meat, 

 yeast). 



(6) In the body : Not found in man, for whom the or- 

 ganism is not pathogenic. It causes mouse septicemia, 

 an artificial infectious disease, discovered by Koch, which 

 also occurred spontaneously in one instance in Greifswald. 



Special Culture Methods. — Inoculation of a white 

 mouse with the suspected material. Stained smear prep- 

 arations, plates, and stab cultures are made from the 

 blood and spleen, where the bacteria are present in abun- 

 dance. 



Pathogenic Effects upon Animals. — Pathogenic 

 (death in two or three days) for house mice (not for field 

 mice). Symptoms : Eyelids stuck together, head drawn in, 

 a tendency to sleep. Also pigeons die in two and one-half 

 to three and one-half days (Th. Smith). Rabbits and 

 guinea-pigs withstand large quantities of bouillon culture. 

 In swine it produces only transitory indisposition. 



Bacterium erysipelatos suum. (Loffler.) Migula. 



(Swine Erysipelas pro parte, Bacillus of Erysipelas of Swine.) 



Bacillus rhusiopathicse suis Kitt. 



Literature.— Loffler (A. G. A. I, 46). Preisz (C. B. xi, 110). 



This organism is very closely related to the one causing 

 mouse septicemia, being identical microscopically, and the 

 stab culture is extremely similar, only the branches are a 

 little more sturdy and bristly (33, ij. When the inocu- 



