334 IMPORTANT VARIETIES OF FISSION-FUNGI. 



Gelatin Stab. — Deep in the gelatin along the stab 

 there occurs first a cloudy growth, then cyst- or tube- 

 shaped excavations, which are filled with cloudy, granular 

 liquid (44, n). 



Agar Plate. — (a) Natural size: Colonies whitish, 

 roundish to ragged, usually surrounded by an exceedingly 

 delicate veil (44, v). 



(b) Magnified sixty times: The original colony appears 

 grayish-yellow, roundish, opaque, surrounded by a broad 

 zone made up of the finest interlacing hairs. Toward the 

 periphery it becomes transparent; toward the center, 

 grayish-yellow and opaque (44, vi). 



Agar Stab. — In the stab prepared by a simple thrust 

 of the platinum loop there develops, deep in the agar, a 

 scaly, ribbon-shaped growth (compare 45, n). If one 

 rotates the loop in the agar, then the growth extends in a 

 wider zone and consists of a cone of cloudy layers (44, i), 

 the surface of which, after a very long time, becomes 

 covered with points and fine serrations (45, in). 



Agar Streak. — No confluent growth, but only single 

 discrete colonies (Votteler). 



Blood-serum. — Sometimes liquefied, sometimes not. 



Bouillon (anaerobic) moderately clouded. 



Milk. — No (according to v. Hibler, very slow) coagu- 

 lation; reaction amphoteric. 



Non-albuminous Nutrient Media. — Upon Uschin- 

 sky's solution no distinct growth. 



Resistant properties of the bacillus are without 

 practical interest, as it sporulates very readily. 



Resistance of the Spores. — See page 331, and Tizzoni 

 and Cattani (C. B. ix, 487). 



Chemical Activities. — See page 331. The forms 

 studied by us form gas from sugar actively; a production 

 of acid could not be demonstrated (on account of simul- 

 taneous abundant formation of alkali). Brain nutrient 

 medium was darkened (v. Hibler). Extremely vigorous 

 production of H 2 S, little indol. Attenuated (slightly 

 virulent) forms, according to Tizzoni and Cattani, often 

 form more acid and grow more luxuriantly (C. B. xi, 150) ; 

 in general, the virulence is w r ell preserved. Upon nutrient 

 media without sugar, we saw no gas production. The 



