384 ACTINOMYCETES. 



segments, and in many cultures present constantly an 

 undoubted, true branching. 



Key to the Recognition of the Most Important Vari- 

 eties of the Genus Corynebacterium. 



1. Plate cultures upon gelatin: Colonies like those of Bact. coli or 

 typhi — i. e. , roundish, and when magnified sixty times, with distinct 

 lineal markings; upon agar and serum-agar, just like Bact. coli. 

 Potato culture brownish-red. Cause of glanders. Corynebact. mallei 

 L. and N., page 384. 



2. Plate cultures upon agar and serum-agar with very characteristic 

 granulation (splintery ! ) . Growth upon potato usually slight. 

 Colorless to yellowish. 



(a) Very luxuriant growth upon the nutrient media, even upon 

 potato. Gelatin gradually discolored brown. Growth often yellowish, 

 sometimes brownish. Not pathogenic for animals. Usually little 

 acid produced in bouillon. Usually no granules in the rods when 

 stained by Neisser's method. Corynebact. pseudodiphtheriticum 

 (Hofmann-Wellenhof ) L. and N., page 404. 



(6) Growth of medium intensity upon agar, and best upon serum- 

 agar; poor upon gelatin and potato. Vigorous production of acid in 

 bouillon. Usually granules in the rods when stained by Neisser's 

 method. Pathogenic for man and animals. Corynebact. diphtheriae 

 Lbffler, L. and N., page 389. 



(c) Scanty growth upon nutrient media. No production of acid in 

 bouillon. No granules staining by Neisser's method. Not patho- 

 genic. Corynebact. xerosis (Neisser and Kuschbert) L. and N., 

 page 406. 



The close relationship of Corynebacterium diphtheriae with Coryn. 

 pseudodiphtheriticum and Coryn. xerosis permits no certain recogni- 

 tion from this key alone. (Compare p. 403. ) 



Corynebacterium mallei. (Loftier and Schutz.) 

 L. and N. 



(Plate 57.) 



Common Names. — Glanders bacillus; German, Rotz; 

 Latin, malleus; French, morve; English, glanders. Bacil- 

 lus mallei Fliigge. 



Principal Literature. — Loffler (A. G. A. I, 141). Kranzfeld (C. B. 

 II, 273). Kitt (C. B. II, 241). 



Microscopic Appearance. — Slender rods (2-3 fi long, 

 0.4 ^ thick), sometimes with brightly shining bodies 

 (metachromatic bodies), which may be shown very well 



