PLATE 44. 

 Bacillus tetani. Nicolaier. (Tetanus Bacillus.) 



I. Sugar-agar stab culture, three days at 37°. 

 II. Sugar-gelatin stab culture, six days at 22°. 



III. Sugar-gelatin plate, four days at 22°. Grown 

 without air. 



IV. Sugar-gelatin plate, four days at 22°. X 60. Nat- 

 ural size. Superficial and deep colonies. Grown without air. 



V. Sugar-agar plate, four days at 37°. Natural size. 

 Grown without air. 



VI. Sugar-agar plate, four days at 37°. X 60. Super- 

 ficial and deep colonies. Grown without air. 



VII. Microscopic preparation. Pure culture on sugar- 

 agar, three days at 37°. X 1000. Bacilli with spores. 

 Double staining according to Ziehl. 



VIII. Microscopic preparation. Pure culture on sugar- 

 agar, two days at 37°. X 1000. Some bacilli contain 

 spores. Stained with fuchsin. 



IX. Microscopic preparation. Pure culture on sugar- 

 agar, twenty-four hours at 37°. X 1000. Extremely long 

 threads with faintly stained intervals. 



X. Microscopic preparation. Pure culture on sugar- 

 agar, six days at 37°. X 1000. Stained with fuchsin. 

 Long threads and chains of spores with faintly stained inter- 

 vals. 



XI. Microscopic preparation. Flagella staining. 

 Highly magnified. 



