PLATE 45- 

 Bacillus Chauvcei. Mace. (Symptomatic Anthrax.) 



I. Sugar-gelatin stab culture, six days at 22°. 

 II. Sugar-agar stab culture, three days at 37°. 



III. Sugar-agar stab culture, three weeks at 37°. 



IV. Sugar-agar plate, four days at 37°. Natural size. 

 Grown as anaerobe. 



V. Sugar-agar plate, four days at 37°. X 60. Super- 

 ficial and deep colony. Grown as anaerobe. 



VI. Sugar-gelatin plate, four days at 22°. Natural size. 

 Grown as anaerobe. 



VII. Sugar-gelatin plate, four days at 22°. X 60. 

 Deep colony, grown as anaerobe. 



VIII. Sugar-gelatin plate, two days at 22°. X 150. 

 Part of a superficial colony. Grown as anaerobe. 



IX. Microscopic preparation. Pure culture on sugar- 

 agar, three days at 37°. X 1000. Bacilli with spores and 

 free spores. Stained with fuchsin. 



