402 DESCRIPTION OF SOME SPECIES OF MILK BACTERIA 



could be seen numerous miliary 

 granulations and a few volumin- 

 ous masses with caseous contents 

 forming as a whole a mass more 

 considerable than the small 

 quantity of butter inoculated (2 

 centimetres cube). Four of these 

 masses, in fact, were larger than 

 a fair-sized nut. The lungs and 

 kidneys, otherwise normal in 

 appearance, showed a few miliary 

 granulations of a similar nature. 

 Both in these miliary granulations 

 and in the caseous masses, of 

 which no detailed histological 

 study was made, were found 

 numerous bacilli presenting all 

 the characters of bacillus tubercu- 

 losis, and in particular when 

 stained by Ehrlich's method 

 (Binot). 



BUTTER BACILLUS (Markl). 

 Source and habitat — Vienna butter. 

 Morphology — Bacilli forming fila- 

 ments, short or long, and resemb- 

 ling streptothrix. 

 Staining properties — Is not very 

 resistant to decolorisation by 

 acids or alcohol. Remains 



coloured by Gram's method. 

 Biology : cultural characteristics. 



Bouillon — Thick surface veil, 

 clear liquid, light precipitate. 



Gelatine plates — Rare, very small, 

 pin - head like colonies. Non- 

 liquefying. 



Agar — On agar plates small 

 colonies of dry appearance and a 

 rose colour. 



Agar stroke — A sparse, dry 

 growth of a rose tint. 



Potato — Rapid growth, dry, and 

 of a rose colour. 



Milk — Surface formation of rose 

 coloured veil. No coagulation. 

 Aerobic. 

 Pathogenic properties — Pure 



cultures inoculated intraperi- 

 toneally into guinea-pigs give rise 

 to subacute hsemorrhagic perito- 

 nitis. Subcutaneous inoculation of 

 a second animal from the lesions 

 of the first, gives rise to an abscess 

 at the point of inoculation. 



BUTTER BACILLUS (Coggi). 



Source — Milan butter. Coggi met 

 with these bacilli in 17-89 per 

 cent, of samples of Milan butter, 

 while 2-12 per cent, only contained 

 true bacilli of tuberculosis. 

 Morphology— ^v!\2iiS. fine rods closely 

 resembling the bacillus of tubercu- 

 losis, always isolated, sometimes a 

 little curved. 

 Motility — Absent. 



Staining properties — They resist de- 

 colorisation by acids, and remain 

 coloured by Gram's method. 

 Biology : cultural characters. 



Bouillon — Formation of a sur- 

 face veil after 48 hours growth. 

 The liquid remains clear with a 

 thread-like precipitate at the bottom 

 of the tube. No indol. 



Gelatine — Surface growth,humid, 

 and of a whitish tint. Round granu- 

 lar colonies, of a yellowish tint, with 

 indented margins. Non-liquefying. 

 Agar — Soft creamy - white 

 growth, turning to a yellowish tint 

 in old cultures. 



Glycerine serum — As on agar, 

 but with the coloration more 

 marked. 



Potato — A moist growth of a 

 greyish tint. 



Milk — A yellowish pellicle forms 

 on the surface of the medium. No 

 coagulation. 



Pathogenic properties — Pure 

 cultures are pathogenic for guinea- 

 pigs when inoculated intraperi- 

 toneally, but the lesions have a 

 very marked tendency to spon- 



