DESCRIPTION OF SPECIES 



405 



Bouillon — A white wrinkled veil, 

 turning to a rose brown. The 

 liquid becomes slightly turbid and 

 emits an unpleasant odour. Indol. 



Gelatine — Growth sparse, non- 

 chromogenic and without wrink- 

 ling. No liquefaction. 



Agar — There is but little growth 

 in the medium. 



Potato — A dr>' brownish yellow 

 growth with ver>' slight wrinkling. 



Milk — Formation of a whitish 

 surface veiL 



Pathogenic properties — This 

 organism, accordmg to Madame 

 Tobler, gives rise in the guinea- 

 pig and the white mouse to a 

 fibrino-purulent peritonitis which 

 is rapidly mortal. The bacilli are 

 found both in the blood and in 

 the glands. 



BUTTER BACILIjUS, No. 8 (Tobler). 



Source and habitat — Zurich butter. 



Morphology — Morphologically re- 

 sembles B. tuberculosis J ramified 

 filaments or club forms are rare. 



Staining reaction — This bacillus has 

 only proved acid-fast when stained 

 by the Ziehl-Neelsen method within 

 the first 2 days of its cultural 

 growth. 



Motility — No motility observed. 



Biology : cultural characters. 



Bouillon — The liquid becomes 

 turbid throughout with surface 

 formation of a pellicle of a pale rose 

 colour which becomes wrinkled and 

 mounts up the sides of the tube. 

 Later a granular deposit forms at 

 the bottom of the tube and the 

 liquid clears itself completely. 



Gelatine — Isolated colonies 

 possess a finely granular centre 

 with a clear bordering zone extend- 

 ing in delicate ramifications into 

 the surrounding medium. 



Glycerine-agar stroke culture — A 

 humid g^owlh turning to a red lead 

 colour at the end of a few days. 



Potato — Humid growth of red 

 lead colour, and feebly wrinkled 

 surface. 



Glycerine potato — In its early 

 stages the growth is creamy, shin- 

 ing, and of a dirty brown colour 

 with no deposit in the liquid, at 

 the end of 6 weeks the culttire is 

 still of a dirty brown tint, shining, 

 and very little raised above the 

 surface of the medium. At times 

 there is a very abundant deposit of 

 a flocculent nature at the bottom of 

 the tube. 



Milk — Milk is not coagulated, 

 but there is surface formation of a 

 I>ellicle of pale rose colour. 



Blood serum — Growth is similar 

 to that upon agar but little less 

 abundant, and the coloiu- is not 

 so deep a red. 



Pathogenic properties — Is patho- 

 genic for guinea-pigs, which, in- 

 jected subcutaneously or intra- 

 peritoneally, die within from 2 to 3 

 weeks, with lesions which are more 

 characteristic when a mixture of 

 the culture with sterilised butter 

 has been injected than when the 

 injection has been with pure cultiu-e 

 alone. 



BUTTER BACILIiUS, No. 4 (Tobler). 



Source and habitat — Zurich butter. 



Morphology— The length of this bacillus 

 is very variable, it is often met 

 with in filaments with ramifications, 

 and in club form. 



Staining reaction — It resists well de- 

 colorisation by acids even in 

 cultures of 3 weeks growth. 



Motility — N on -motile. 



Biology: cultural characters — Accord- 

 ing to Madame Tobler cultures 

 resemble very much those of No. 



