4i8 DESCRIPTION OF SOME SPECIES OF MILK BACTERIA 



Motility — Non-motile. 



Spore formation — Absent. 



Biology : cultural characters {charac- 

 ters of cultures). 



Bouillon — Upon bouillon a light 

 yellowish veil with deposit at the 

 bottom of the tube of a viscous 

 nature and yellowish tint. 



Glycerine bouillon — In glycerine 

 bouillon a thick veil, dry, white, 

 and folded. The liquid remains 

 clear with an abundant white 

 deposit. 



Gelatine — Abundant culture of a 

 creamish-white colour ; no wrink- 

 ling. The medium is not liquefied. 



Agar — Upon agar stab-culture, 

 a round greyish growth with raised 

 edges and umbilical centre. Stroke 

 cultures upon agar give after from 

 24 to 36 hours a smooth, creamy, 

 glistening growth, slightly greyish, 

 and non - wrinkled. The culture 

 changes but little with age. 



Glyceritte-agar—\] Y>or). glycerine- 

 agar a thick, white, brilliant growth 

 with deposit in the water of con- 

 densation, which is soon covered 

 with a veil adherent to the walls 

 of the tube. Later on the growth 

 becomes slightly folded. At the 

 age of about 3 weeks the culture 

 is creamy, presenting a few, light 

 folds, and of yellow-orange colour. 

 In older cultures the growth is 

 very abundant, raised considerably 

 above the surface, and irregularly 

 folded and convoluted, the colour 

 varying in depth from light to dark 

 orange or even red brick. 



Glycerine potato — Culture at first 

 of a dull, white appearance, becom- 

 ing later creamy and glistening, 

 with wrinkling on surface as growth 

 proceeds, the liquid in the bulb of 

 tube is covered with a surface veil, 

 and there is deposit in the bottom 

 of the tube. 



Milk — Is not coagulated, but at 

 the end of 3 weeks has a dirty 

 yellowish-brown appearance. 



Blood serum — Small, shining, 

 transparent colonies : no coloration. 



Anaerobic or aerobic — It is a 

 facultative anaerobic, but the 

 growth in hydrogen is slight. 



Tefnperature of development — 

 Optimum temperature of develop- 

 ment 37° C. 



Pathogenesis — Subcutaneous or 

 intraperitoneal injection of pure 

 cultures produce only an abscess 

 at site. If injected with butter 

 itself in white mice, granulations 

 are produced in thoracic and 

 abdominal viscera, showing no 

 giant cells, but commencing casea- 

 tion {see Plate 24, p. 254). 



BACILLUS PRIBURGBNSIS, No. 2 



(Korn). 



Source and habitat — Butter of Friburg. 



Morphology — Small rods, one to three 

 times longer than broad, often of 

 irregular form and clubbed, but 

 without ramifications. 



Staining — Stains well after the method 

 of Ziehl-Neelsen, and is much less 

 sensible to decolorisation by sul- 

 phuric acid than Korn No. i. 

 Stains fairly well with ordinary 

 aniline dyes and holds the Gram. 



Spore formation — No spore formation 

 observed. 



Motility — Non-motile. 



Biology : cultural characters — Com- 

 pared with otheracid-fast organisms, 

 this organism grows but poorly 

 upon ordinary laboratory media, 

 and gives scarcely any growth at 

 all at room temperature. 



Bouillon — The liquid becomes 

 troubled and of muddy appearance, 

 and there is a slight brownish de- 

 posit at the bottom of the tube. 

 No indol. 



