DESCRIPTION OF SPECIES 



419 



Glycerine bouillon — In glycerine 

 bouillon, the liquid remains clear, 

 with slight surface veil and fine 

 whitish deposit at the bottom of 

 the tube. 



Gelatine — Ver>' scanty growth 

 with dull wrinkled surface. Growth 

 does not increase with age. 



Agar — Upon agar the growth 

 appears in about 24 hours. The 

 culture is creamy, shining, and 

 of a yellowish to deep yellow tint. 



Glycerine-agar — On glycerine- 

 agar as upon agar. 



Glycerine potato — Very little 

 growth, a few yellowish colonies 

 are occasionally observed. 



Milk — Is not coagulated. After 

 about 3 weeks, the medium becomes 

 of a diry rose colour, and a crust 

 forms on the surface of the liquid 

 which later on becomes of a dirty 

 yellowish colour. 



Blood serum — Upon blood serum 

 a brilliant culture of light orange 

 colour. 



Temperature of development — 

 Optimum temperature 37° C. 



Pathogenesis — WTiite mice, 

 chickens, pigeons, and guinea-pigs 

 not affected by inoculating pure 

 culture. Rabbits are not refractory. 

 Lesions appear similar to tuber- 

 culosis {see Plate 24, p. 254). 



GRASS BACHiliUS, No. 2 (Moeller). 



Source and habitat — On certain 

 grasses, hay-dust, etc. Thence 

 to milk. 



Morphology — Rods ; i to 5 ^ long, -2 

 to -4 /i broad ; variable forms, 

 filaments, club - shaped forms, 

 branching. 



Staining Reaction. — Acid-fast ; Ziehl- 

 Neelsen ; also Gram. 



J/<?/////)/— Absent. 



Biology: cultural characters {includ- 

 ing biochemical features'). 



Bouillon — In 3 to 4 days at 22° C. 

 a flaky deposit and a surface mem- 

 brane ; no general turbidity and 

 no odour ; more abundant growth 

 in glycerine bouillon. 



Gelatine — In 4 to 5 days thick, 

 white growth, becoming yellow in 

 older parts of culture. No lique- 

 faction. 



Agar — On glycerine-agar, rapid 

 growth ; small colonies becom- 

 ing confluent, yellow ; slightly 

 raised. 



Potato — Abundant growth at 

 37° C. ; thick, moist, white. 



Milk — Rapid growth, changing 

 medium to brown-red colour, slight 

 pellicle. No coagfulation. Milk 

 becomes acid in 2 to 3 days. 



Blood serum — Small, meagre 

 colonies ; undeveloped. 



Aerobic. 



Pathogenesis — Similar to 

 Timothy grass bacillus. Moeller 

 states : " The bacillus produces the 

 same lesions as the butter bacillus ; 

 the cultures in milk are the most 

 virulent." 



BACTT.TiT OP GUHiliBBBAU, 



Bacillus " a " of Gulllebeaa — Found in 

 the milk of cows suffering from 

 mastitis ; motile ; 1-2 m long by i 

 M broad. Resembles in growth 

 the B. cerogenes. Does not stain 

 by Gram's method. Causation, 

 according to Freudenreich, of 

 abnormal blowing in cheese. 

 Rapid coagulation of milk, and 

 fermentation of lactose. Gelatine 

 liquefied. Colonies spherical and 

 granular. In gelatine, growth along 

 track of needle and white patch 

 on surface. On agar, growth is 

 white, and on potato yellowish and 

 viscid. 



