DESCRIPTION OF SPECIES 



437 



solved, the senim shows a green 

 fluorescence. 



Aerobic. 



Non-pathogenic. 



No. 2 differs only from No. i in 

 that the elements are thicker, and 

 the viscosity produced in milk and 

 other liquids is not so pronounced. 



CLOSTRIDIUM PCBTTDUM (Liborius). 

 Source and habitat — Soil and milk. 



Morphology ; form — Bacillus. Rods 

 I ju broad, singly and in filaments. 



Staining reaction — Ordinary stains. 



Motility — Active. 



spore formation — Present, resembling 

 that of B. butyricusj oval, central, 

 or terminal. 



Biology: cultural characters {includ- 

 ing biochemical features). 



Gelatine plates and tubes — Gela- 

 tine is rapidly liquefied. Inocu- 

 lated in the depth the liquefac- 

 tion spreads from below upwards. 

 There is much gas formation with 

 unpleasant odour, and splitting up 

 of the medium. Small irregular 

 colonies. 



Agar plates and tubes — The 

 colonies form branching processes 

 resembling those of B, cedematis 

 maligni; colonies small, yeUow, 

 and irregiilar in shape ; gas forma- 

 tion. 



Blood serum — Homogeneous 

 cloudiness, and at end of pimcture 

 a few colonies. 



Anaerobic. 



Non-pathogenic. 



LEUCONOSTOC MESENTEROIDES 



(Crenkowsky and Van Tieghem). 



Morphology J form — Cocci and rods 

 singly and in chains ; and in 

 zoogloea surrounded by a thick 

 gelatinous envelope. The spores, 



1-3 to 2 At in diameter are of rotmd 

 or ellipsoidal form, with thick 

 membrane and glistening contents. 

 In liquid media formation of small 

 masses, very adherent. The zoogloea 

 masses are of almost cartilaginous 

 consistency. Particularly well culti- 

 vated in liquid media containing 

 glucose. Spores have been demon- 

 strated by Van Tieghem. Aerobic. 



MIOROCOOCUS ACXDI LACTIS 



(Kriiger). 



Source and habitat — Milk and butter. 



Morphology — Oval coccus ; lo m to 

 i'5 ft. diameter ; dippococci or 

 tetrads. 



staining reaction — Ordinary aniline 

 stains. 



Biology : cultured characters {includ- 

 ing biochemical features). 



Gelatinepiates and tubes — Round, 

 white colonies with ragged border; 

 liquefying ; granular, white growth 

 in stab - culture, liquefies in i to 

 3 days. A wTinkled pellicle floats 

 on surface of liquefied gelatine. 



Agar plates and tubes — Growth 

 on surface becomes wrinkled, 

 tenacious, and sticky ; develops a 

 yellow-sahnon colour. 



Potato — An abundant growth, 

 folded, and of a salmon colour. 



Milk — Forms lactic acid from 

 milk sugar. Milk coagulated into 

 a hard clot in 5 days (15^-35' C.). 

 Eventually, albuminous bodies are 

 peptonised, with production of 

 sticky character and a pasty 

 odour. Yellow lumps of fat 

 appear on the surface. Butyric 

 acid and alcohol are found to be 

 present. 



Anaerobic or Aerobic — A facul- 

 tative anaerobe. 



Non-pathogenic. 



