110 THE BACTERIOPHAGE 



infection. It has been isolated from the blood of three fowls 

 which were recovering from the infection. Outside of epizootic 

 foci it has been found in the intestine of healthy animals. 



Bad. diphtheriae 



Two strains of bacteriophage active for only atoxic strains of 

 Bad. diphtheriae have been isolated from the feces of two horses 

 immunized by the injection of cultures of diphtheria bacilli. 

 This observation is only mentioned. Lack of opportunity has 

 prevented further examination of these strains, a study which 

 certainly offers much of interest. 



Staphylococcus 



A bacteriophage active for the staphylococcus has been isolated 

 under the following circumstances. A guinea pig bit my left 

 index finger and on the next day an inflammation appeared which 

 persisted for three days. On the fourth day there was an acciimu- 

 lation of pus, of which about ten drops were secured. When 

 planted directly upon agar there developed one colony of Sta- 

 phylococcus albus and six of Staphylococcus aureus. The remainder 

 of the pus was mixed with twenty cubic centimeters of bouillon 

 and placed in the incubator for 24 hours, then filtered through 

 infusorial earth and a bougie. After five passages at the expense 

 of Staphylococcus albus lysis was secured. At first the bacterio- 

 phage isolated did not show any activity in vitro for Staphylococ- 

 cus aureus. It has been possible to develop a virulence for this 

 organism only after about fifty passages in a mixed Staphylococ- 

 cus aureus and Staphylococcus albus suspension, following the 

 technic previously indicated for the enhancement of the latent 

 virulence of an anti-staphylococcus bacteriophage toward B. 

 dysenteriae Shiga. 



Bacterium of barbone 



About thirty strains of bacteriophage for this organism have 

 been isolated, of which twelve were extremely active. Their 

 activity was comparable when tested against different bacterial 

 strains, some derived from Italy, others f rom Indo-China. We 



