IMMUNIZATION BY MEANS OF THE BACTERIOPHAGE 



251 



Two control steers died in 22 and 26 hours after the inoculation, and one 

 buffalo, as control, died in 19 hours. 



C. Twenty animals; 5 steers, 3 young buffaloes, and 12 adult buffaloes 

 were tested during the period from the twenty-first to the sixtieth day after 

 the immunizing injection. All received 1000 surely fatal doses of culture. 

 All resisted without showing any reaction. Five control animals died, 

 all between 16 and 23 hours after the inoculation of culture. 



IV. Eight steers received 0.04 cc. of bacteriophage culture. They 

 were tested after a variable number of days by the inoculation of 5 surely 

 fatal doses of virulent culture. The results were: 



The last steer, No. 102, was tested 60 days after the injection of the immun- 

 izing dose by the inoculation of 50 surely fatal doses of culture. It resisted 

 without showing any disturbance. 



V. A last experiment, as a control, was performed with a view to testing 

 the practical application of immunization of buffaloes against barbone by 

 M. Le Louet after my departure from Saigon. Twelve steers received by 



