270 THE BACTERIOPHAGE 



where the bacterium is known and cultivable. It is only neces- 

 sary to apply the principles which have been discussed in the 

 course of this work. 10 



10 Since the publication of the French edition of this work Bruynoghe 

 and Maisin at the Bacteriologic Institute of Louvain, and Beckerich and 

 Hauduroy at the Institute of Hygiene at Strasbourg have confirmed these 

 conclusions. 



Bruynoghe and Maisin have treated with success affections due to the 

 staphylococcus and anthrax by the subcutaneous injection of cultures of the 

 bacteriophage. 



Beckerich and Hauduroy have experimented with typhoid fever and with 

 pyelocystitis. They have employed cultures of the bacteriophage virulent 

 for the causative bacterium, heated to 58°C. for thirty minutes. They 

 record the following cases; 



1. An adult with typhoid fever of moderate severity. The patient 

 ingested 2 cc. of bacteriophage culture on the 18th day of the disease. 

 Defervescence took place forty-eight hours later. 



2. An adult with typhoid fever of moderate severity. The patient 

 received the same treatment, with the same results. The culture was 

 given on the ninth day of the disease. 



3. An infant with severe typhoid fever. The patient was given 2 cc. 

 of the bacteriophage culture by mouth and in addition the simultaneous 

 injection of 1 cc. This treatment was given on the twentieth day. The 

 temperature came down within forty-eight hours and the apyrexia was 

 permanent. 



4. An infant with a paratyphoid B infection, whose condition was grave. 

 On the ninth day the bacteriophage was given by the simultaneous inges- 

 tion and injection of the culture. After the next day the apyrexia was 

 permanent. 



5. An infant with paratyphoid B infection of average severity. On 

 the 23rd day the bacteriophage was given by ingestion and injection. 

 The apyrexia was permanent after the next day. 



Two adults affected with typhoid fever of the ataxo-adynamic form and 

 with pronounced myocardial involvement were treated. The apyrexia 

 which followed the treatment within forty-eight hours did not prevent 

 death. Considering that in these two cases the failure might be due, either 

 to a too delayed intervention, or to too small a dose in view of the severity 

 of the cases, they decided to increase the quantity of bacteriophage culture 

 administered by ingestion in such cases. 



6. An infant affected with typhoid fever in very severe form. On the 

 tenth day the patient was given 5 cc. of culture by mouth and a simul- 

 taneous injection of 1 cc. Within forty-eight hours there was permanent 

 defervescence with euphoria. 



7. An infant with a very severe case of typhoid fever. The same treat- 

 ment was given on the fourteenth day. Defervescence with euphoria 

 occurred in forty-eight hours. 



