INFECTIVE INFLAMMATION. 75 



inoculated with pus ; the micrococcus was exactly similar to the 

 staphylococcus albus, for which it was first mistaken ; later, it was 

 shown that it was not a pus-microbe, as it did not liquefy gelatin. 

 All of the animals recovered. Two rabbits inoculated with an 

 entire culture of yellow sarcinse upon agar, mixed with one-tenth of 

 a drop of croton oil, also recovered. The experiments with patho- 

 genic microbes always produced positive results. Three series, 

 with three separate microorganisms, were carried out. The staphy- 

 lococcus pyogeues aureus grown from osteomyelitic pus was first 

 used. In seventeen out of forty-one experiments, this microbe 

 alone was used ; in eleven it was mixed with croton oil, in six with 

 trypsin, and in seven with agar-agar. From injections with the 

 staphylococcus alone, the following results were obtained : 



1. Large quantities produced fibrinous suppurative peritonitis. 



2. In quantities of two plate cultures, four rabbits succumbed 

 to suppurative peritonitis. 



3. Half of this quantity produced the same results. 



4. In yet smaller quantities, still the same result, and peritonitis 

 only failed to develop when a very minute quantity of the culture 

 was used. 



In all cases in which peritonitis was produced, inoculations of 

 the products of inflammation upon nutrient media yielded positive 

 results. In hardened specimens of the peritoneum, stained with 

 various coloring agents, the microorganisms could be seen in the 

 lymph spaces. The suppurative nature of the peritonitis thus 

 induced became more apparent the longer life was prolonged. An 

 entire agar culture of the bacillus pyocyaneus caused death in from 

 twenty-four to forty-eight hours. The autopsy revealed a fibriuous 

 hemorrhagic peritonitis. The exudation consisted largely of red 

 corpuscles and a large unmber of bacilli. Pure cultures of the 

 bacillus could be obtained by inoculating the fluid upon agar-agar. 

 One-fifth of this quantity proved harmless. 



M jln hardened sections, the bacilli were found in the lymph spaces 

 of the central tendon of the diaphragm, the parietal peritoneum, 

 visceral peritoneum, and in the capsule of the spleen and liver, also 

 in the uriniferous tubules and Malpighian bodies of the kidney. 

 The next series of experiments was made to ascertain what caused 

 the inflammation in cases of perforative peritonitis. The fresh 

 intestinal contents of a healthy animal, just killed, were divided 

 into three parts, one of which was at once injected into several 

 rabbits without filtration, in doses of one syringefnl. The second 

 portion was filtered, and of the filtrate two and a half to three and 

 a half syriugefuls were injected into each rabbit; the third portion 

 was sterilized according to Tyndall's directions for eight days, and 

 then one syringeful was injected into each animal. The results 



