84 SUPPURATION. 



a transient redness ; when larger quantities are used some authors 

 claim they produce small circumscribed abscesses. If a pure cul- 

 ture is injected into a serous cavity, it causes, first, inflammation, 

 and, later, effusion, which is again absorbed. 



9. BACILLUS PYOGENES FCETIDUS. Passet found this organism 

 (Fig. 3, Passet) in the pus of a peri-rectal abscess This bacillus 

 grows on gelatin, forming a delicate white or grayish layer on the 

 surface, but causes no liquefaction. When cultivated on agar-agar 

 and potato it has the appearance of a light brown glistening layer, 

 which emits a very offensive odor. In milk this smell is not pro- 

 duced. This organism is not pathogenic in rabbits. In mice 

 traces of the culture do no harm ; the injection of several drops 

 causes septica3mia. Injection of about ten minims of the cultiva- 

 tion into guinea-pigs causes an abscess in which the bacilli alone 

 are found ; direct injection into the circulation causes sepsis. 



FIG. 4. 



Bacillus pyogenes foetidus, 700 : 1. Bacillus pyocyaneus, 700 : 1. 



(PASSET.) (FLUGGE.) 



10. BACILLUS PYOCYANEUS. Ernst found in blue pus two 

 kinds of bacilli, Fig. 4 (Fliigge), which he designated as bacillus 

 pyocyaneus. Ledderhose (" Ueber den blauen Eiter," Deutsche 

 Zeitschrift /. Chirurgie, B. xviii. Heft 3), by extensive cultivations 

 of these bacilli, obtained a considerable quantity of pyocyanin and 

 by chemical analysis determined its formula to be C 14 H 14 N 2 C. In 

 doses of one gramme as muriate of pyocyanin injected into the 

 circulation of different animals, he observed no toxic symptoms. 

 When a pure culture of the bacilli was injected, he observed in- 

 flammation and suppuration, and attributes this result, not to the 

 presence of pyocyanin, but to other as yet unknown phlogistic and 

 pyogenic substances. 



Experimental and Clinical Evidences which Prove that Pus-microbes 

 are the Only and Essential Cause of Suppuration. 



Rosenbach found that in dogs and rabbits a small quantity of a 

 pure culture of the staphylococcus pyogenes aureus injected under 

 the skin produced a most violent suppurative inflammation ; cul- 



