86 SUPPUEATION. 



animals. Garre (" Zur Aetiologie der acut eitrigen Eutziiuduu- 

 geu," Fortschritte der Medicin, 1885, No. 6) made a superficial 

 abrasion on one of his fingers and applied a pure culture of the 

 staphylococcns pyogenes aureus ; the only symptom observed was 

 a slight redness eighteen to twenty-four hours after the inoculation. 

 He then made three small incisions and inoculated himself with a 

 larger quantity of the culture, which was followed by a slight sub- 

 epidermal suppuration. The same author made numerous cultiva- 

 tions from pus of different forms of acute abscesses, and always 

 succeeded in demonstrating the presence of one or more of the pus- 

 microbes. From the product of acute, diffuse phlegmouous inflam- 

 mation the culture consisted usually of streptococci. 



Fehleisen repeated the same experiments with cultures of the 

 different kinds of pus-microbes, and, if he succeeded in causing 

 suppuration, this was always very slight. He also found minute 

 doses administered subcutaneously harmless, while a larger quantity 

 of pus- microbes suspended in water, almost without exception, 

 caused abscesses, and, in animals, very large doses produced death 

 from sepsis before suppuration could take place. 



Bockhardt introduced a trace of the mixed cultivation of staphy- 

 lococcus aureus and albus into the cutis of his left forefinger ; after 

 forty-eight hours a small abscess had formed, and was opened, and 

 the pus contained the staphylococcus pyogenes aureus. 



He also made on himself endermic inoculations with a mixture 

 of staphylococcus pyogenes aureus and albus. Fourteen hours after 

 injection there were at the seat of inoculation, in a space about the 

 size of a five-shilling piece, twenty-five impetigo pustules, varying 

 from the size of a pin's head to that of a lentil, a few, but not the 

 majority, of these being traversed by hair. They contained the 

 cocci employed. Bumm injected pure cultivations of staphylococcus 

 pyogeues aureus into the subcutaneous tissue of his own arm and 

 into the arms of two other persons. In each instance an abscess 

 developed, which varied from the size of a pigeon's egg to that of 

 a man's fist, according to the time which elapsed before they were 

 opened, and these abscesses contained the same species of microbes 

 as were injected. 



Fehleisen ( u Zur Aetiologie der Eiteruug, Arbeiteu aus der 

 Chirurgischen Klinik der Konigl. Universitat," Berlin, Dritter 

 Theil., 1887), who believes that the ptomaines produced by the pus- 

 microbes are the direct cause of suppuration, has also made numer- 

 ous experiments on animals by injecting pure cultures, or pus. In 

 order to ascertain whether the presence of atmospheric air had 

 anything to do with the formation of pus, he made subcutaneous 

 sections of muscles and tendons with a tenotomy knife which had 

 been dipped in a mixture of water and pus-microbes, and, as sup- 



