INOCULATION EXPERIMENTS. 159 



Nocard et Roux (" Sur la culture du bacille de la Tuberculose," 

 Annales de V Institute Pasteur, i. 1887, No. 1, pp. 19-29) have 

 found that coagulated blood -serum is improved for the growth of 

 the bacillus of tuberculosis by adding peptone, soda, and sugar. 

 A further addition of 6 to 8 per cent, of glycerin favors the 

 growth of the bacillus still more, while at the same time it prevents 

 the formation of a crust upon the culture medium, which otherwise 

 forms by evaporation. They also made successful cultivations 

 upon agar-agar bouillon, to which was added 6 to 8 per cent, of 

 glycerin, kept at a temperature of 39 C. (102.2 F.). 



INOCULATION EXPERIMENTS Even before the discovery of the 

 bacillus of tuberculosis by Koch, genuine tuberculosis was produced 

 in animals by inoculation with the products of what was then 

 described as scrofula. Hueter inoculated the anterior chamber of 

 the eye in rabbits with lupus tissue and produced tuberculosis of 

 the iris. Schiiller ( Untersuchung uber die Entstehung und Ursache 

 der scrofidosen und tuberculosen Getenldeiden, 1880) introduced 

 fragments of lupus tissue into the veins of animals, and in this 

 way produced pulmonary tuberculosis. He also claimed to have 

 discovered the microbe of tuberculosis by fractional cultivation from 

 lupus tissue which, when conveyed into the vessels of the lungs, 

 produced phthisis, and when injected into joints tubercular inflam- 

 mation, caseation, and, finally, miliary tuberculosis. Koch (Mit- 

 theilungen aus dem Kaiserlichen Gesundheitsamte, B. xi. 1883) 

 inoculated the anterior chamber of the eyes of eighteen rabbits 

 from five cases of lupus, and in all of them tuberculosis of the iris 

 was produced, and, if life was prolonged for a sufficient length of 

 time, was followed by tuberculosis of the lymphatic glands of the 

 neck, lungs, kidneys, liver, and spleen. Similar results were 

 obtained in five guinea-pigs. 



Cornet has recently made numerous experiments in Koch's 

 laboratory on animals to ascertain the inoctilability of tuberculosis 

 through abrasions of the skin, He found that if lupus tissue, or 

 a pure culture of tubercle bacilli, is applied to a cutaneous abrasion, 

 the result in most, if not in all, cases is a local tuberculosis in the 

 adjacent lymphatic glands, and, later, a general miliary tuberculosis. 



The same author (" Demonstration von tuberculosen Driisen- 

 Schwellungen nach Impfuugen von Tuberkel bacillen bei Hunden," 

 Centralblatt f. d. Gesammte Medicin, No. 29, 1889) made subse- 

 quently a long series of experiments on dogs to ascertain the differ- 

 ent avenues through which infection is known to take place. 

 Tuberculous sputum and pure cultures inserted into the lower 

 conjunctival sac in healthy dogs produced tissue hyperplasia at the 

 seat of inoculation and was followed by infection of the cervical 

 glands on the corresponding side. Some of the glands had under- 



