210 GLANDERS. 



progressed quite rapidly to a fatal termination. Abscesses were 

 frequently found in the testicle and the epididymis in the male, and 

 in the breast and external genital organs of the female. The face, 

 nasal cavity, and ankle-joint were also frequently the seat of nlcer- 

 ative processes. If the disease proved fatal, death usually occurred 

 three or four weeks after the inoculation. At the post-mortem, 

 aside from the affections which have been enumerated, nodules were 

 found in the spleen, lungs, and often also in the liver. Field mice 

 proved a great deal more susceptible to the virus of glanders, than 

 guinea-pigs, as they usually died three or four days after inocula- 

 tion. The post-mortem in these animals showed at the point of 

 inoculation an infiltration from which swollen lymphatic vessels 

 led to the nearest lymphatic glands. In the spleen and liver, 

 which were always greatly enlarged, numerous small nodules were 

 found, while the remaining internal organs presented a normal 

 appearance. Glanders in guinea-pigs and field mice presents a 

 series of pathological changes which cannot be mistaken for any 

 other affection. The bacilli of glanders in the different organs can 

 be detected most readily in recent specimens. In the blood bacilli 

 were found only in very acute cases, a circumstance which explains 

 why so many inoculations with the blood of glanderous horses 

 proved unsuccessful. 



Lundgreu (" Forsok till remodliug af rots-mikrobeu," Hygiea, 

 Bd. xlix., Heft 2, S. 91) took a nodule from the lungs of a horse 

 which had died of glanders and implanted fragments of it under 

 the skin of rabbits. The animals died about the nineteenth day 

 after the inoculation, and the necropsy revealed induration and 

 small abscesses at the point of infection, and small yellow nodules 

 in the spleen, liver, lungs, testicles, and mucous membrane of the 

 nose. These tissues stained with methyl-blue showed the bacilli of 

 glanders. Implantations of spleen tissue into other rabbits fixed 

 the period of incubation in this animal at from eleven to twelve days. 



At a recent meeting of the Academy of Medicine, M. Cornil 

 (British Medical Journal, June 7, 1890) gave an account of M. 

 Babes' s researches on the bacillus of glanders, which show that this 

 microorganism, when obtained from pure cultivations, can penetrate 

 the healthy tissue of animals, and thus cause glanders. Rubbing 

 in an ointment containing the bacillus rarely succeeds, and only 

 when the virus is very active, and obtained from a perfectly fresh 

 cultivation. M. Nocard has repeated these experiments, and two 

 guinea-pigs out of five were infected by this method of inoculation. 



Kranzfeld (Zur Kenntniss des Rotz-bacillus," Gentralblatt fur 

 jBakteriologie und Parasitenkunde, Bd. xi., No. 10) has recently 

 published the results which he obtained by inoculations with the 

 virus of glanders in an animal which had not hitherto been sub- 

 jected to experimentation of this kind. He procured a pure cul- 



