240 GONORRHCEA. 



regards Gram's method of staining as necessary to make the micro- 

 scopic examination of gonorrhoea! pus of diagnostic value, as the 

 gouococcus is not stained by this method, while nearly all other 

 diplocoeci found in the urethra are colored thereby. 



These conclusions, which agree with those of Roux, are the 

 result of the examination of 86 patients with acute and chronic 

 gonorrhoea. The almost entire certainty of this test is rendered 

 absolute by the observation of the further characteristic of the 

 gonococci, namely, that they are found within the pus-corpuscles. 



Sternberg (The Medical News, January 20, 1883) cultivated a 

 micrococcus from gonorrhoeal discharges in bouillon which in its 

 morphology resembled the gonococcus described by Neisser. He 

 made numerous inoculation experiments in animals and a few in 

 man with only negative results. Subcutaneous injections of a pure 

 culture also proved harmless. He came to the conclusion that the 

 micrococcus which he found corresponded to the micrococcus urea3 

 of Cohn ; the pathogenic eifect of which has been shown to be the 

 cause of the alkaline fermentation of urine (Pasteur). 



Leistikow (Deutsche Medicinal-Zeitung, September 7, 1882) has 

 observed that during the first stage of gonorrhoea, when the dis- 

 charge is thick and abundant, but few gonococci could be found. 

 They were found abundant in the thin and scanty secretion of the 

 later stages, sometimes even when the disease had existed for a 

 year. All authorities who have studied the relations of the gono- 

 coccus to gonorrhoea with the greatest care, insist that, for diagnostic 

 purposes, it is not only necessary to demonstrate its presence, but 

 to ascertain its intra-cellular location and the manner in which this 

 microbe arranges itself in groups in the protoplasm of the cell be- 

 tween the nucleus and the envelope of the cell, and these conditions 

 should be studied with the greatest care in all medico-legal cases in 

 which a positive opinion must rest on a microscopical examination 

 of the secretion. 



