BACTERIOLOGICAL STUDY OF SYPHILIS. 243 



zone had formed around the streak made by the needle, which on 

 microscopical examination was seen to be composed exclusively of 

 small bacilli. Embedded in the culture, granules were also found, 

 which were thought might be spores. Similar results have since 

 been obtained by Martineau and Hammonic (Les Baderies, par 

 Coruil et Babes, p. 774) and Birch-Hi rschfeld (Centralblatt f. d. 

 med. Wiss., 1882, Nos. 33, 44), who cultivated the bacillus from 

 the primary lesion, condylomata, and gummata of internal organs. 



INOCULATION EXPERIMENTS. The transmission of syphilis to 

 animals by implantation of syphilitic tissue, or by inoculations with 

 cultures, has, so far, not met with uniform success, as it has been 

 found difficult to find animals susceptible to syphilitic infection. 



Kleb's experiments on monkeys yielded positive results. Mar- 

 tineau and Hammonic experimented on the same animal, and pro- 

 duced the disease by inoculation with a pure culture. They observed 

 twenty-eight days after inoculation of the prepuce two indurations 

 which were followed by general secondary symptoms. In one case 

 in which Klebs used a culture for inoculation an abscess formed, and 

 the animal remained apparently well until the seventh week, when a 

 granulation-swelling formed at the base of the upper jaw which 

 ulcerated, and from this point a cheesy infiltration extended to the 

 base of the skull ; the same deposits were found between the base 

 of the skull and the dura mater. From the abscess which still 

 contained cheesy masses lymphatic infection had taken place, and 

 the glands also contained cheesy material. No miliary tuberculosis 

 could be found. Bacilli were found in the cheesy material which 

 were identical with those contained in the inoculation material. In 

 another case a piece of tissue from a hard chancre was implanted 

 under the skin of one of the posterior extremities. Six weeks after 

 the implantation general and febrile symytoms supervened, attended 

 by a papular eruption on the forehead and face. Death occurred 

 five months after inoculation, and at the post-mortem syphilitic 

 lesions were found in the skull and lungs. 



Bacteriological and Experimental Study of Syphilis. 



Doutrelepont and Schiitz (" Die Bacillen bei Syphilis," Deutsche 

 med. Wochenschrift, 1885, No. 9) are more reserved in their state- 

 ments than Lustgarten in reference to the causation of syphilis by 

 the bacillus just described. In regard to the uniform presence of 

 a bacillus in syphilitic lesions they concur in the views advanced 

 by Lustgarten. They found the bacillus between and within cells. 

 They consider it as the probable cause of syphilis, but they thought 

 that further proof must be furnished by culture and inoculation 

 experiments before this question could be definitely settled. 



