180 Sterilization and Disinfection 



28). Here, under a pressure of two or three atmospheres, 

 sufficient heat is generated to immediately destroy the 

 spores. 



Because of its convenience many laboratory workers 



Fig. 29. Pasteur-Chamberland filter arranged to filter under 

 pressure. 



habitually use the autoclave for the sterilization of all 

 media not injured by the high temperature. The steriliza- 

 tion, to be complete, requires that the exposure shall be 

 for fifteen minutes at 110 C. (six pounds pressure). 



