246 The Determination of Bacteria 



0. Gelatin colonies roundish, not distinctly ameboid. 



* Gelatin not liquefied. 



t Decolorized by Gram's method. 



Gas .generated in glucose bouillon. 

 Milk coagulated. COLON GROUP. 

 Milk not coagulated. HOG CHOLERA GROUP. 

 No gas generated in glucose bouillon. TYPHOID 



GROUP. 

 ft Stained by Gram's method. B. MURIPESTIFER 



GROUP. 

 ** Gelatin liquefied. 



t Gas generated in glucose bouillon. B. CLOACA GROUP. 



ft No gas generated in glucose bouillon. Include a 



large number of bacteria not sufficiently described 



to arrange in groups. 



6. Gelatin colonies ameboid, cochleate, or otherwise irregular. 



* Gelatin liquefied. PROTEUS VULGARIS GROUP. 

 ** Gelatin not liquefied. B. ZOPFI GROUP. 



II. Produce endospores. 



A. Aerobic and facultative anaerobic. 



1. Rods not swollen at sporulation. 



a. Gelatin liquefied. 



* Liquefaction of the gelatin takes place slowly. Fer- 

 ment urea, with strong production of ammonia. 

 URO-BACILLUS GROUP OF MIQUEL. 

 ** Gelatin liquefied rather quickly. 



t Potato cultures rugose. POTATO BACILLUS GROUP. 

 tf Potato cultures not distinctly rugose. B. SUBTILIS 



GROUP. 

 6. Gelatin not liquefied. B. SOLI GROUP. 



2. Rods spindle-shaped at sporulation. B. LICHENIFORMIS 



GROUP. 



3. Rods clavate at sporulation. B. SUBLANATUS GROUP. 



B. Obligate anaerobic. 



1. Rods not swollen at sporulation. MALIGNANT EDEMA 



GROUP. 



2. Rods spindle-shaped at sporulation. CLOSTRIDIUM GROUP. 



3. Rods clavate-capitate at sporulation. TETANUS GROUP. 



PSEUDOMONAS (Migula). 



I. Cells colorless, without a red-colored plasma and without sulphur 



granules. 



A. Grow in ordinary culture media. 

 1. Without endospores. 

 a. Aerobic and facultative anaerobic. 



* Without pigment. 



t Gelatin not liquefied. 



Gas generated in glucose bouillon. Ps. MONADI- 



FORMIS GROUP. 

 No gas generated in glucose bouillon. Ps. AM- 



BIGUA GROUP. 

 ft Gelatin liquefied. 



Gas generated in glucose bouillon. Ps. COADU- 



NATA GROUP. 

 No gas generated in glucose bouillon. Ps. FAIR- 



MONTENSIS GROUP. 



* Produce pigment on gelatin or agar. 

 f Pigment yellowish. 



