Pathogenesis 317 



In speaking about this mixture Dr. Torrey told me that 

 the exact reaction was its most important feature, as other- 

 wise the gonococci soon died. Tubes of about 12 cm. of 

 the mixture were heated to about 60 C. for several hours 

 and then tested for sterility. The cocci were cultivated at 

 3 6 -37 C. After eighteen to twenty-four hours' incubation 

 a slight granular growth appears near the surface and on 

 the sides of the tube. This slowly increases until after six 

 days the medium is well clouded on shaking. Large rab- 

 bits were used for making the serum, and were intraperi- 

 toneally inoculated with 10 c.c. of an entire culture. The 

 first . inoculation resulted in a loss of weight, sometimes 

 amounting to one-fourth of the body-weight. After an 

 interval of five or six days a second injection is given; then 

 after a similar interval, a third, and so on. The best results 

 were obtained when cultures from six to fifteen days old 

 were employed. The rabbits were bled for the first time 

 after the sixth dose, as if the treatment be pushed, they 

 soon fall into a state of cachexia, rapidly emaciate, and die. 

 Each animal furnishes 70-90 cm. of the serum, which was 

 inclosed in 2 -cm. bulbs, hermetically sealed, and kept without 

 any preservative. 



With serum made in this way by Torrey, Rogers* treated 

 a number of obstinate cases of gonorrheal rheumatism, with 

 most satisfactory results. 



Good results in gonorrheal arthritis and in gleet are also 

 claimed for treatment with gonococco-vaccines prepared as 

 suggested by A. E. Wright. 



* "Jour. Amer. Med. Assoc.," XLVI, p. 261, Jan. 27, 1906. 



