Metabolic Products 



417 



Milk. In litmus milk the glanders bacillus produces acid. 

 A firm coagulum forms and subsequently separates from 

 the clear reddish whey. 



Metabolic Products. Mallein. Babes,* Bonome,f 

 Pearson, J and others have prepared a substance, mallein, 

 from cultures of the glanders bacillus, and have employed 

 it for diagnostic purposes. It seems to be useful in veter- 

 inary medicine, the reaction following its injection into 

 glandered animals being similar to that caused by the in- 

 jection of tuberculin into tuberculous animals. The 



Fig. 122. Culture of glanders bacillus upon cooked potato (Loffler). 



preparation of mallein is simple. Cultures of the glanders 

 bacillus are grown in glycerin bouillon for several weeks, 

 and killed by heat. The culture is then filtered through 

 porcelain, to remove the dead bacteria, and evaporated 

 to one-tenth of its volume. It has also been prepared 

 from potato cultures, which are said to yield a stronger 

 product. The agent is employed exactly like tuberculin, 

 the temperature being taken before and after its hypodermic 



* " Archiv de Med. exp. et d'Anat. patholog.," 1892, No. 4. 



f " Deutsche med. Wochenschrift," 1894, Nos. 36 and 38, pp. 703, 

 725, and 744. 



J"Jour. of Comp. Med. and Vet. Archiv.," Phila., xn, 1891, pp. 

 411-415. 



27 



