Cultivation 511 



each organism is provided with a single flagellum situated 

 at its end, and is actively motile. Although at first thought 

 to be a variety of the cholera spirillum, marked differences 

 of growth were soon observed, and showed the organism 

 to be a separate species. 



Staining. The organism stains readily with the ordinary 

 solutions, but not by Gram's method. 



Cultivation. Colonies. The growth upon gelatin 

 plates is rapid, and leads to such extensive liquefaction 

 that four or five dilutions must frequently be made to se- 

 cure few enough organisms to enable one to observe the 



>-** * ^ ^ ^J**r-*2* I V 



Fig. 151. Spirillum of Kinkier and Prior, from an agar-agar culture. 

 X 1000 (Itzerott and Niemann). 



growth of a single colony. To the naked eye the deep colo- 

 nies appear as small white points (Fig. 152)- They rapidly 

 reach the surface, begin liquefaction of the gelatin, and by 

 the second day appear about the size of lentils, and are 

 situated in little depressions. Under the microscope they 

 are yellowish-brown, finely granular, and are surrounded 

 by a zone of sharply circumscribed liquefied gelatin. Careful 

 examination with a high-power lens shows rapid movement 

 of the granules in the colony. 



Gelatin Punctures. In gelatin punctures the growth 

 takes place rapidly along the whole length of the puncture, 

 forming a stocking-shaped liquefaction filled with cloudy 



