(4) 



cent solution of formaldehyde, tne bias tome res stood out al- 

 most as distinctly as in tne liviUi, material. The larger 

 species of the two Actino t -"ochae , founa in Beaufort Harbor is 

 much more active than the other, and v/hen it comes in contact 

 with the fixing, fluid, the preoral lobe is bent upward into an 

 unusi'9.1 positiori. Consequently a few drops of four per cent 

 solution, of muriate of cocaine in fifty per cent alcohol was 

 added to the v/ater containing the Actinot rochae. After this 

 treatment the Actino tro chae died in their usual form when put 

 in the fixing fluid. ■Plemning" fluid as well as the acetic 

 sublimate was found to be a \er-j valuable fixin^ agent fb r the 

 Actino tro chae, Keidenhain's iron haematoxylin was used in 

 staining sections o^ the adult and a secondary stain of alco- 

 holic eosin or rubin gave verv good results. The most sat- 

 isfactor-y stain for sections of young larvae aiid Actinotroch- 

 ae was found to be a solution of saffranin in anilin water. 

 Since it was very desirable to make a study of tiie adults 

 throughout the year aijd as i t v/as not possible to reinain in 

 Beaufort for this purpose aurin.^ the winter and spring months, 

 the author nad sfecimena collected and sent to Johns Hopkins 

 University at dif-^erent times. Here they were platted in aqua- 

 ria filled with sea water, v/hich '.vas kept in tjood conaition by 

 a rich growth yf diatoms on top of a layer of sana, ITot only 



